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N4TG3
N4TG3
规格:
货期:
编号:TS258962
品牌:Testobio
产品名称: N4TG3
商品货号: TS258962
<strong>Cell line </strong>: N4TG3
DSMZ no. : TS258962
Species : Mus musculus origin confirmed by COI DNA barcoding
Cell type : neuroblastoma
Origin : 6-thioguanine resistant mutant of cell line N4 that was established from a mouse of strain A/J with neuroblastoma C1300; cells were described in the literature to form increased levels of cAMP in response to prostaglandin E1 which is inhibited by opiate receptors; cells are aneuploid (modal chromosome number of 86)
Reference(s) : 14355, 15020, 14355
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: flat cell body, slightly adherent, occasionally with short processes; image; image
Medium : 90% Dulbeccos MEM + 10% h.i. FBS + 100 µM 6-thioguanine
Subculture : split saturated culture 1:2 to 1:4 every 2-4 days (cells grow slightly adherent, but can be detached without trypsin)
Incubation : at 37 °C with 10% CO2
Harvest : about 10-20 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization assays
Fingerprint : unique Hinf I-(gtg)5 DNA profile
Viruses : ELISA: reverse transcriptase positive
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N4TG3

  • 货号: TS258962
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  • 品牌 : TESTOBIO
产品名称: N4TG3
商品货号: TS258962
<strong>Cell line </strong>: N4TG3
DSMZ no. : TS258962
Species : Mus musculus origin confirmed by COI DNA barcoding
Cell type : neuroblastoma
Origin : 6-thioguanine resistant mutant of cell line N4 that was established from a mouse of strain A/J with neuroblastoma C1300; cells were described in the literature to form increased levels of cAMP in response to prostaglandin E1 which is inhibited by opiate receptors; cells are aneuploid (modal chromosome number of 86)
Reference(s) : 14355, 15020, 14355
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: flat cell body, slightly adherent, occasionally with short processes; image; image
Medium : 90% Dulbeccos MEM + 10% h.i. FBS + 100 µM 6-thioguanine
Subculture : split saturated culture 1:2 to 1:4 every 2-4 days (cells grow slightly adherent, but can be detached without trypsin)
Incubation : at 37 °C with 10% CO2
Harvest : about 10-20 x 106 cells/80 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization assays
Fingerprint : unique Hinf I-(gtg)5 DNA profile
Viruses : ELISA: reverse transcriptase positive
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