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IEC-6
IEC-6
规格:
货期:
编号:TS258971
品牌:Testobio
产品名称: IEC-6
商品货号: TS258971
<strong>Cell line </strong>: IEC-6
DSMZ no. : TS258971
Species : confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding
Cell type : normal small intestine
Origin : normal rat epithelial cells from the small intestine; cells were described in the literature to synthesize fibronectin and collagen; no continuous cell line: about 10-15 doublings after thawing of the stock cells
Reference(s) : 14866
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: adherent, transparent, epitheloid cells growing as monolayers (polymorphy of cell forms)
Medium : 45% Dulbeccos MEM (4.5 g/L glucose) and 45% RPMI 1640 + 10% h.i. FBS + 0.1 U/ml insulin (human or rat)
Subculture : split confluent cultures 1:2 to 1:6 about twice a week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2
Incubation : at 37 °C with 5% CO2
Doubling time : about 50 hours
Harvest : about 16 x 106 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses : ELISA: reverse transcriptase negative, SMRV -
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IEC-6

  • 货号: TS258971
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: IEC-6
商品货号: TS258971
<strong>Cell line </strong>: IEC-6
DSMZ no. : TS258971
Species : confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding
Cell type : normal small intestine
Origin : normal rat epithelial cells from the small intestine; cells were described in the literature to synthesize fibronectin and collagen; no continuous cell line: about 10-15 doublings after thawing of the stock cells
Reference(s) : 14866
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: adherent, transparent, epitheloid cells growing as monolayers (polymorphy of cell forms)
Medium : 45% Dulbeccos MEM (4.5 g/L glucose) and 45% RPMI 1640 + 10% h.i. FBS + 0.1 U/ml insulin (human or rat)
Subculture : split confluent cultures 1:2 to 1:6 about twice a week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2
Incubation : at 37 °C with 5% CO2
Doubling time : about 50 hours
Harvest : about 16 x 106 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses : ELISA: reverse transcriptase negative, SMRV -
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