| 产品名称: | IEC-6 |
|---|---|
| 商品货号: | TS258971 |
| <strong>Cell line </strong>: | IEC-6 |
| DSMZ no. : | TS258971 |
| Species : | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type : | normal small intestine |
| Origin : | normal rat epithelial cells from the small intestine; cells were described in the literature to synthesize fibronectin and collagen; no continuous cell line: about 10-15 doublings after thawing of the stock cells |
| Reference(s) : | 14866 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | adherent, transparent, epitheloid cells growing as monolayers (polymorphy of cell forms) |
| Medium : | 45% Dulbeccos MEM (4.5 g/L glucose) and 45% RPMI 1640 + 10% h.i. FBS + 0.1 U/ml insulin (human or rat) |
| Subculture : | split confluent cultures 1:2 to 1:6 about twice a week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | about 50 hours |
| Harvest : | about 16 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses : | ELISA: reverse transcriptase negative, SMRV - |
| 产品名称: | IEC-6 |
|---|---|
| 商品货号: | TS258971 |
| <strong>Cell line </strong>: | IEC-6 |
| DSMZ no. : | TS258971 |
| Species : | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type : | normal small intestine |
| Origin : | normal rat epithelial cells from the small intestine; cells were described in the literature to synthesize fibronectin and collagen; no continuous cell line: about 10-15 doublings after thawing of the stock cells |
| Reference(s) : | 14866 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | adherent, transparent, epitheloid cells growing as monolayers (polymorphy of cell forms) |
| Medium : | 45% Dulbeccos MEM (4.5 g/L glucose) and 45% RPMI 1640 + 10% h.i. FBS + 0.1 U/ml insulin (human or rat) |
| Subculture : | split confluent cultures 1:2 to 1:6 about twice a week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | about 50 hours |
| Harvest : | about 16 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses : | ELISA: reverse transcriptase negative, SMRV - |