F9
F9
规格:
货期:
编号:TS258974
品牌:Testobio
| 产品名称： | F9 |
|---|---|
| 商品货号： | TS258974 |
| <strong>Cell line </strong>： | F9 |
| DSMZ no. ： | TS258974 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | embryonal carcinoma |
| Origin ： | initiated from a testicular teratocarcinoma of a strain 129 mouse |
| Reference(s) ： | 14386, 14989 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells with undifferentiated morphology growing in clumps |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | presoak flasks with 0.1% gelatin at 4 °C for 2 hours, wash 3x with PBS; split confluent culture 1:10 to 1:20 every 2-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | about 70 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near-diploid karyotype with 6% tetraploidy - 38(37-40)<2n> - 1-2 centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
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周雪
13372109301
| 产品名称： | F9 |
|---|---|
| 商品货号： | TS258974 |
| <strong>Cell line </strong>： | F9 |
| DSMZ no. ： | TS258974 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | embryonal carcinoma |
| Origin ： | initiated from a testicular teratocarcinoma of a strain 129 mouse |
| Reference(s) ： | 14386, 14989 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells with undifferentiated morphology growing in clumps |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | presoak flasks with 0.1% gelatin at 4 °C for 2 hours, wash 3x with PBS; split confluent culture 1:10 to 1:20 every 2-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | about 70 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near-diploid karyotype with 6% tetraploidy - 38(37-40)<2n> - 1-2 centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
