| 产品名称: | XC |
|---|---|
| 商品货号: | TS258989 |
| <strong>Cell line </strong>: | XC |
| DSMZ no. : | TS258989 |
| Species : | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type : | sarcoma |
| Origin : | the original tumor was induced in a Wistar rat by intramuscular injection of Rous sarcoma virus; cells have been used as indicator cells for detection of murine leukemia viruses |
| Reference(s) : | 14997, 14670, 14904 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelial-like, small cells growing as monolayers |
| Medium : | 90% MEM (with Earles salts) + 10% h.i. FBS + non-essential amino acids |
| Subculture : | split confluent culture 1:4 every 3 days using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Harvest : | about 1-2 x 107 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Fingerprint : | unique Hinf I-(gtg)5 DNA profile |
| Viruses : | ELISA: reverse transcriptase positive; PCR: SMRV - |
| 产品名称: | XC |
|---|---|
| 商品货号: | TS258989 |
| <strong>Cell line </strong>: | XC |
| DSMZ no. : | TS258989 |
| Species : | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type : | sarcoma |
| Origin : | the original tumor was induced in a Wistar rat by intramuscular injection of Rous sarcoma virus; cells have been used as indicator cells for detection of murine leukemia viruses |
| Reference(s) : | 14997, 14670, 14904 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelial-like, small cells growing as monolayers |
| Medium : | 90% MEM (with Earles salts) + 10% h.i. FBS + non-essential amino acids |
| Subculture : | split confluent culture 1:4 every 3 days using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Harvest : | about 1-2 x 107 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Fingerprint : | unique Hinf I-(gtg)5 DNA profile |
| Viruses : | ELISA: reverse transcriptase positive; PCR: SMRV - |