CX-1
CX-1
规格:
货期:
编号:TS259015
品牌:Testobio
| 产品名称： | CX-1 |
|---|---|
| 商品货号： | TS259015 |
| <strong>Cell line </strong>： | CX-1 |
| DSMZ no. ： | TS259015 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | colon adenocarcinoma (derivative of HT-29) |
| Origin ： | established from the tumor tissue of a 44-year-old woman with well-differentiated colon adenocarcinoma in 1984; cell line is a subclone of cell line HT-29 (DSM ACC 299) |
| Reference(s) ： | 14569 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small circular cells, adherent, grow to large colonies in which the single cells are no longer discernible |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA, seed out about 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | about 30 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 14% polyploidy - 72(65-74)<3n>XX, -X, +4, +7, -8, -8, -9, +11, +11, -13, +15, +17, -18, +2mar, der(4)t(1;4)(p11;p15)t(1;?) (p32-36;?), del(4)(q32), del(7)(p21), i(13q), add(15)(p11), del(17)(p12), add(21)(p11) - two small markers present - carries 3 markers similar to those present in HT-29 - rearrangements of chs 1, 7, 17 typical of colon carcinoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | CX-1 |
|---|---|
| 商品货号： | TS259015 |
| <strong>Cell line </strong>： | CX-1 |
| DSMZ no. ： | TS259015 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | colon adenocarcinoma (derivative of HT-29) |
| Origin ： | established from the tumor tissue of a 44-year-old woman with well-differentiated colon adenocarcinoma in 1984; cell line is a subclone of cell line HT-29 (DSM ACC 299) |
| Reference(s) ： | 14569 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small circular cells, adherent, grow to large colonies in which the single cells are no longer discernible |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA, seed out about 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | about 30 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 14% polyploidy - 72(65-74)<3n>XX, -X, +4, +7, -8, -8, -9, +11, +11, -13, +15, +17, -18, +2mar, der(4)t(1;4)(p11;p15)t(1;?) (p32-36;?), del(4)(q32), del(7)(p21), i(13q), add(15)(p11), del(17)(p12), add(21)(p11) - two small markers present - carries 3 markers similar to those present in HT-29 - rearrangements of chs 1, 7, 17 typical of colon carcinoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
