EM-3
EM-3
规格:
货期:
编号:TS259021
品牌:Testobio
| 产品名称： | EM-3 |
|---|---|
| 商品货号： | TS259021 |
| <strong>Cell line </strong>： | EM-3 |
| DSMZ no. ： | TS259021 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | chronic myeloid leukemia in blast crisis |
| Origin ： | established from the bone marrow of a 5-year-old Caucasian girl with Philadelphia chromosome-positive CML (chronic myeloid leukemia) in second relapse in 1980 (after bone marrow transplantation day +47); sister cell line of EM-2 (DSM ACC 135) |
| Reference(s) ： | 14488, 14656, 14883 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to oval cells in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.5-1.5 x 106 cells/ml; subculture at a 1:2 to 1:3 dilution every 48 hours; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | grows up to about 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR - |
| Fingerprint ： | same DNA profile as EM-2 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype - 73(69-77)<3n>X, -X, -X, t(9;22)(q34;q11), i(17q) |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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李晶晶
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周雪
13372109301
| 产品名称： | EM-3 |
|---|---|
| 商品货号： | TS259021 |
| <strong>Cell line </strong>： | EM-3 |
| DSMZ no. ： | TS259021 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | chronic myeloid leukemia in blast crisis |
| Origin ： | established from the bone marrow of a 5-year-old Caucasian girl with Philadelphia chromosome-positive CML (chronic myeloid leukemia) in second relapse in 1980 (after bone marrow transplantation day +47); sister cell line of EM-2 (DSM ACC 135) |
| Reference(s) ： | 14488, 14656, 14883 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round to oval cells in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.5-1.5 x 106 cells/ml; subculture at a 1:2 to 1:3 dilution every 48 hours; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | grows up to about 2 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, HLA-DR - |
| Fingerprint ： | same DNA profile as EM-2 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype - 73(69-77)<3n>X, -X, -X, t(9;22)(q34;q11), i(17q) |
| Molec. Genetics ： | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
