KB
KB
规格:
货期:
编号:TS259023
品牌:Testobio
| 产品名称： | KB |
|---|---|
| 商品货号： | TS259023 |
| <strong>Cell line </strong>： | KB |
| DSMZ no. ： | TS259023 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | cervix carcinoma (derivative of HELA) |
| Origin ： | originally described as "epidermoid carcinoma established from the mouth of a Caucasian man in 1954"; however, as shown by DSMZ DNA fingerprinting (see below) this cell line must be considered an HELA subclone (human cervix carcinoma, see HELA DSM ACC 57) |
| Reference(s) ： | 14499, 14710, 14709 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells, epithelial-like, growing as monolayers; image; image; image |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + MEM non-essential amino acids + 0.075% Na-bicarbonate |
| Subculture ： | split confluent culture 1:3 to 1:5 every 3-6 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | about 5-10 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA as HELA using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype with 8% polyploidy - 76(73-78)<3n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | KB |
|---|---|
| 商品货号： | TS259023 |
| <strong>Cell line </strong>： | KB |
| DSMZ no. ： | TS259023 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | cervix carcinoma (derivative of HELA) |
| Origin ： | originally described as "epidermoid carcinoma established from the mouth of a Caucasian man in 1954"; however, as shown by DSMZ DNA fingerprinting (see below) this cell line must be considered an HELA subclone (human cervix carcinoma, see HELA DSM ACC 57) |
| Reference(s) ： | 14499, 14710, 14709 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells, epithelial-like, growing as monolayers; image; image; image |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + MEM non-essential amino acids + 0.075% Na-bicarbonate |
| Subculture ： | split confluent culture 1:3 to 1:5 every 3-6 days using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | about 5-10 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA as HELA using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype with 8% polyploidy - 76(73-78)<3n> |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/II -, MLV -, SMRV - |
