A-10
A-10
规格:
货期:
编号:TS259024
品牌:Testobio
| 产品名称： | A-10 |
|---|---|
| 商品货号： | TS259024 |
| <strong>Cell line </strong>： | A-10 |
| DSMZ no. ： | TS259024 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | smooth muscle |
| Origin ： | established from the thoracic aorta of a DB1X embryonic rat in 1976; cells were described in the literature to display properties characteristic of smooth muscle cells |
| Reference(s) ： | 14662 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large, epithelial-like, some fibroblast-like, adherent cells growing in monolayers; image; image; image; image |
| Medium ： | 80% Dulbeccos MEM + 20% h.i. FBS |
| Subculture ： | seed out at 1 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA; after thawing, cells may contain a significant amount of granulae; confluent cultures look more transparent |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 70-100 hours |
| Harvest ： | about 1-2 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Cytogenetics ： | rat karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | A-10 |
|---|---|
| 商品货号： | TS259024 |
| <strong>Cell line </strong>： | A-10 |
| DSMZ no. ： | TS259024 |
| Species ： | confirmed as rat by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | smooth muscle |
| Origin ： | established from the thoracic aorta of a DB1X embryonic rat in 1976; cells were described in the literature to display properties characteristic of smooth muscle cells |
| Reference(s) ： | 14662 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | large, epithelial-like, some fibroblast-like, adherent cells growing in monolayers; image; image; image; image |
| Medium ： | 80% Dulbeccos MEM + 20% h.i. FBS |
| Subculture ： | seed out at 1 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA; after thawing, cells may contain a significant amount of granulae; confluent cultures look more transparent |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 70-100 hours |
| Harvest ： | about 1-2 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Fingerprint ： | unique Hinf I-(gtg)5 DNA profile |
| Cytogenetics ： | rat karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
