UT-7
UT-7
规格:
货期:
编号:TS259035
品牌:Testobio
| 产品名称： | UT-7 |
|---|---|
| 商品货号： | TS259035 |
| <strong>Cell line </strong>： | UT-7 |
| DSMZ no. ： | TS259035 |
| Species ： | confirmed as human with IEF of LDH, NP |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7) at diagnosis in 1988; cells are constitutively cytokine-dependent and responsive to various cytokines. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14482, 14686, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round (relatively large) cells growing singly in suspension (ca. 1-2% cells are slightly adherent); image; image; image; image |
| Medium ： | 80% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 5 ng/ml GM-CSF |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; cultivate in 12- or 24-well-plate for optimal growth; split saturated culture 1:2 every 2-3 days. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | saturation density at ca. 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, cyCD68 +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype - 72-88<4n>XXYY, +1, -8, -9, -9, -9, -11, -11, -12, -13, -13, -14, -18, -21, -22, +6mar, del(1)(q42)x2, t(2;4)(p16-23;q27-31)x1-2, der(2)t(2;5)(p16-23;q12-14)x1-2, add(5)(q11), del(5)(q13), der(6;13)(p10;q10), i(7q)x1-2, add(8)(q24)x1-3, del(9)(q22), add(12)(p12-13), der(13)t(13;13)(p11;q14), add(14)(p11), add(15)(p11), add(16)(p1?), add(17)(p11), add(19)(p13)x2, add(19)(q13), i(21q)x1-2 - extensive subclonal variation present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | UT-7 |
|---|---|
| 商品货号： | TS259035 |
| <strong>Cell line </strong>： | UT-7 |
| DSMZ no. ： | TS259035 |
| Species ： | confirmed as human with IEF of LDH, NP |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7) at diagnosis in 1988; cells are constitutively cytokine-dependent and responsive to various cytokines. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14482, 14686, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round (relatively large) cells growing singly in suspension (ca. 1-2% cells are slightly adherent); image; image; image; image |
| Medium ： | 80% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 5 ng/ml GM-CSF |
| Subculture ： | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; cultivate in 12- or 24-well-plate for optimal growth; split saturated culture 1:2 every 2-3 days. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70 hours |
| Harvest ： | saturation density at ca. 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, cyCD68 +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype - 72-88<4n>XXYY, +1, -8, -9, -9, -9, -11, -11, -12, -13, -13, -14, -18, -21, -22, +6mar, del(1)(q42)x2, t(2;4)(p16-23;q27-31)x1-2, der(2)t(2;5)(p16-23;q12-14)x1-2, add(5)(q11), del(5)(q13), der(6;13)(p10;q10), i(7q)x1-2, add(8)(q24)x1-3, del(9)(q22), add(12)(p12-13), der(13)t(13;13)(p11;q14), add(14)(p11), add(15)(p11), add(16)(p1?), add(17)(p11), add(19)(p13)x2, add(19)(q13), i(21q)x1-2 - extensive subclonal variation present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
