SP2/0-AG14
SP2/0-AG14
规格:
货期:
编号:TS259043
品牌:Testobio
| 产品名称： | SP2/0-AG14 |
|---|---|
| 商品货号： | TS259043 |
| <strong>Cell line </strong>： | SP2/0-AG14 |
| DSMZ no. ： | TS259043 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | myeloma |
| Origin ： | SP2/0-AG14 is a hybrid between BALB/c spleen cells and the myeloma cell line P3X63AG8; cells were described as not synthesizing or secreting immunoglobulin chains, being resistant to azaguanine at 20 µg/ml, and not growing in HAT medium; cell line is used as a fusion partner for the production of hybridomas secreting specific monoclonal antibodies |
| Reference(s) ： | 14828, 14959 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; image; image |
| Medium ： | 92.5% Iscoves MDM + 7.5% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | maintain at 0.1-0.5 x 106 cells/ml; split 1:3 to 1:5 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml; viability may be low after thawing and during the first few days, but cells recover after about one week |
| Incubation ： | at 37 °C with 7.5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximal density at 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
SP2/0-AG14
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | SP2/0-AG14 |
|---|---|
| 商品货号： | TS259043 |
| <strong>Cell line </strong>： | SP2/0-AG14 |
| DSMZ no. ： | TS259043 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | myeloma |
| Origin ： | SP2/0-AG14 is a hybrid between BALB/c spleen cells and the myeloma cell line P3X63AG8; cells were described as not synthesizing or secreting immunoglobulin chains, being resistant to azaguanine at 20 µg/ml, and not growing in HAT medium; cell line is used as a fusion partner for the production of hybridomas secreting specific monoclonal antibodies |
| Reference(s) ： | 14828, 14959 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; image; image |
| Medium ： | 92.5% Iscoves MDM + 7.5% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | maintain at 0.1-0.5 x 106 cells/ml; split 1:3 to 1:5 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml; viability may be low after thawing and during the first few days, but cells recover after about one week |
| Incubation ： | at 37 °C with 7.5% CO2 |
| Doubling time ： | ca. 30-40 hours |
| Harvest ： | maximal density at 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
