BPH-1
BPH-1
规格:
货期:
编号:TS259046
品牌:Testobio
| 产品名称： | BPH-1 |
|---|---|
| 商品货号： | TS259046 |
| <strong>Cell line </strong>： | BPH-1 |
| DSMZ no. ： | TS259046 |
| Species ： | originally confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | benign prostate hyperplasia |
| Origin ： | prostate epithelial cells from a 68-year-old man with benign prostate hyperplasia; cells were immortalized with SV-40 large T-antigen; cells were described in the literature to express cytokeratins 8, 18, and 19 (but not 14) and to metabolize prostatic androgens |
| Reference(s) ： | 14602 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing as monolayers; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 20 ng/ml testosterone + 5 µg/ml transferrin + 5 ng/ml sodium selenite + 5 µg/ml insulin |
| Subculture ： | seed out at 0.5-1 x 106 cells in a 80cm2 flask; split confluent culture 1:3 to 1:8 every 3-7 days using trypsin/EDTA (cells should be incubated for more than 10 min with trypsin/EDTA at 37 °C) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 3 x 106 cells/80cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 2.5% polyploidy - 75(69-79)<3n>XXY, -4, +5, +6, +7, -10, +11, -12, +15, +21, +3mar - +dmin - i(3q), add(7)(p11), i(8q), add(10)(p13) - sideline with add(13)(p12) and smaller 10p+ marker - matches published karyotype - carries large submetacentric 10p+ marker |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | BPH-1 |
|---|---|
| 商品货号： | TS259046 |
| <strong>Cell line </strong>： | BPH-1 |
| DSMZ no. ： | TS259046 |
| Species ： | originally confirmed as human with IEF of AST, LDH, NP |
| Cell type ： | benign prostate hyperplasia |
| Origin ： | prostate epithelial cells from a 68-year-old man with benign prostate hyperplasia; cells were immortalized with SV-40 large T-antigen; cells were described in the literature to express cytokeratins 8, 18, and 19 (but not 14) and to metabolize prostatic androgens |
| Reference(s) ： | 14602 |
| Biosafety level ： | 1, GMO-S1 |
| Risk assessment ： | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing as monolayers; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 20 ng/ml testosterone + 5 µg/ml transferrin + 5 ng/ml sodium selenite + 5 µg/ml insulin |
| Subculture ： | seed out at 0.5-1 x 106 cells in a 80cm2 flask; split confluent culture 1:3 to 1:8 every 3-7 days using trypsin/EDTA (cells should be incubated for more than 10 min with trypsin/EDTA at 37 °C) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 3 x 106 cells/80cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 2.5% polyploidy - 75(69-79)<3n>XXY, -4, +5, +6, +7, -10, +11, -12, +15, +21, +3mar - +dmin - i(3q), add(7)(p11), i(8q), add(10)(p13) - sideline with add(13)(p12) and smaller 10p+ marker - matches published karyotype - carries large submetacentric 10p+ marker |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
