| 产品名称: | NEURO-2A |
|---|---|
| 商品货号: | TS259054 |
| <strong>Cell line </strong>: | NEURO-2A |
| DSMZ no. : | TS259054 |
| Species : | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type : | neuroblastoma |
| Origin : | established from the spontaneous tumor of a strain A albino mouse |
| Reference(s) : | 14667, 14826 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | about 30% of the cells grow like neuronal cells; 70% are round, loosely attached cells; image; image ; image |
| Medium : | 90% Dulbeccos MEM + 10% h.i. FBS + 1x non-essential amino acids |
| Subculture : | split culture 1:4 about once a week using trypsin/EDTA, cells do not grow to confluence and grow rather slowly; seed out at ca. 1 x 106 cells/25 cm2 |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 70 hours |
| Harvest : | about 8 x 106 cells/75 cm2; maximal density at ca. 20 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses : | ELISA: reverse transcriptase positive; PCR: SMRV - |
| 产品名称: | NEURO-2A |
|---|---|
| 商品货号: | TS259054 |
| <strong>Cell line </strong>: | NEURO-2A |
| DSMZ no. : | TS259054 |
| Species : | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type : | neuroblastoma |
| Origin : | established from the spontaneous tumor of a strain A albino mouse |
| Reference(s) : | 14667, 14826 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | about 30% of the cells grow like neuronal cells; 70% are round, loosely attached cells; image; image ; image |
| Medium : | 90% Dulbeccos MEM + 10% h.i. FBS + 1x non-essential amino acids |
| Subculture : | split culture 1:4 about once a week using trypsin/EDTA, cells do not grow to confluence and grow rather slowly; seed out at ca. 1 x 106 cells/25 cm2 |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | ca. 70 hours |
| Harvest : | about 8 x 106 cells/75 cm2; maximal density at ca. 20 x 106 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses : | ELISA: reverse transcriptase positive; PCR: SMRV - |