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NEURO-2A
NEURO-2A
规格:
货期:
编号:TS259054
品牌:Testobio
产品名称: NEURO-2A
商品货号: TS259054
<strong>Cell line </strong>: NEURO-2A
DSMZ no. : TS259054
Species : Mus musculus origin confirmed by COI DNA barcoding
Cell type : neuroblastoma
Origin : established from the spontaneous tumor of a strain A albino mouse
Reference(s) : 14667, 14826
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: about 30% of the cells grow like neuronal cells; 70% are round, loosely attached cells; image; image ; image
Medium : 90% Dulbeccos MEM + 10% h.i. FBS + 1x non-essential amino acids
Subculture : split culture 1:4 about once a week using trypsin/EDTA, cells do not grow to confluence and grow rather slowly; seed out at ca. 1 x 106 cells/25 cm2
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 70 hours
Harvest : about 8 x 106 cells/75 cm2; maximal density at ca. 20 x 106 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses : ELISA: reverse transcriptase positive; PCR: SMRV -
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NEURO-2A

  • 货号: TS259054
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  • 品牌 : TESTOBIO
产品名称: NEURO-2A
商品货号: TS259054
<strong>Cell line </strong>: NEURO-2A
DSMZ no. : TS259054
Species : Mus musculus origin confirmed by COI DNA barcoding
Cell type : neuroblastoma
Origin : established from the spontaneous tumor of a strain A albino mouse
Reference(s) : 14667, 14826
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: about 30% of the cells grow like neuronal cells; 70% are round, loosely attached cells; image; image ; image
Medium : 90% Dulbeccos MEM + 10% h.i. FBS + 1x non-essential amino acids
Subculture : split culture 1:4 about once a week using trypsin/EDTA, cells do not grow to confluence and grow rather slowly; seed out at ca. 1 x 106 cells/25 cm2
Incubation : at 37 °C with 5% CO2
Doubling time : ca. 70 hours
Harvest : about 8 x 106 cells/75 cm2; maximal density at ca. 20 x 106 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses : ELISA: reverse transcriptase positive; PCR: SMRV -
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