BA-F8
BA-F8
规格:
货期:
编号:TS259059
品牌:Testobio
| 产品名称： | BA-F8 |
|---|---|
| 商品货号： | TS259059 |
| <strong>Cell line </strong>： | BA-F8 |
| DSMZ no. ： | TS259059 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | this hybridoma cell line was described in the literature to secrete a mouse monoclonal antibody (IgG2b) that reacts with rat type 1 fibers in immunofluorescence and with rat type 1 MHCs (myosin heavy chain) in Western blotting; the cell line was established in 1986 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse that had been immunized with purified myosin from bovine atrium |
| Reference(s) ： | 14401 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells) |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine |
| Subculture ： | split saturated culture 1:3 to 1:4 every 2-3 days; seed out at ca. 0.2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | maximal density at about 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG2 monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid/pentaploid karyotype - 92(89-99)<4n> - 1-3 centric fusion markers and 1-3 dmin present in most cells |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
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周雪
13372109301
| 产品名称： | BA-F8 |
|---|---|
| 商品货号： | TS259059 |
| <strong>Cell line </strong>： | BA-F8 |
| DSMZ no. ： | TS259059 |
| Species ： | Mus musculus origin confirmed by COI DNA barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | this hybridoma cell line was described in the literature to secrete a mouse monoclonal antibody (IgG2b) that reacts with rat type 1 fibers in immunofluorescence and with rat type 1 MHCs (myosin heavy chain) in Western blotting; the cell line was established in 1986 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse that had been immunized with purified myosin from bovine atrium |
| Reference(s) ： | 14401 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells) |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine |
| Subculture ： | split saturated culture 1:3 to 1:4 every 2-3 days; seed out at ca. 0.2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | maximal density at about 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG2 monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid/pentaploid karyotype - 92(89-99)<4n> - 1-3 centric fusion markers and 1-3 dmin present in most cells |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
