| 产品名称: | AM-C6SC8 |
|---|---|
| 商品货号: | TS259067 |
| <strong>Cell line </strong>: | AM-C6SC8 |
| DSMZ no. : | TS259067 |
| Species : | originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding |
| Cell type : | normal kidney cells |
| Origin : | derived as a subclone from the cell line AM II which was established from a normal porcine kidney in 1983; cells have been used in assays to test toxins and mutagens |
| Reference(s) : | 15163 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelial-like cells growing in monolayers; image; image |
| Medium : | 80-90% Medium 199 (Hanks salts) + 10-20% h.i. FBS |
| Subculture : | cells are difficult to culture and grow slowly to very slowly! split confluent culture 1:5 to 1:10 about once a week using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Harvest : | cell harvest of about 8 x 106 cells/80 cm2 or 1 x 107 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses : | ELISA: reverse transcriptase positive; PCR: SMRV - |
| 产品名称: | AM-C6SC8 |
|---|---|
| 商品货号: | TS259067 |
| <strong>Cell line </strong>: | AM-C6SC8 |
| DSMZ no. : | TS259067 |
| Species : | originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding |
| Cell type : | normal kidney cells |
| Origin : | derived as a subclone from the cell line AM II which was established from a normal porcine kidney in 1983; cells have been used in assays to test toxins and mutagens |
| Reference(s) : | 15163 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | epithelial-like cells growing in monolayers; image; image |
| Medium : | 80-90% Medium 199 (Hanks salts) + 10-20% h.i. FBS |
| Subculture : | cells are difficult to culture and grow slowly to very slowly! split confluent culture 1:5 to 1:10 about once a week using trypsin/EDTA |
| Incubation : | at 37 °C with 5% CO2 |
| Harvest : | cell harvest of about 8 x 106 cells/80 cm2 or 1 x 107 cells/175 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses : | ELISA: reverse transcriptase positive; PCR: SMRV - |