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AM-C6SC8
AM-C6SC8
规格:
货期:
编号:TS259067
品牌:Testobio
产品名称: AM-C6SC8
商品货号: TS259067
<strong>Cell line </strong>: AM-C6SC8
DSMZ no. : TS259067
Species : originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding
Cell type : normal kidney cells
Origin : derived as a subclone from the cell line AM II which was established from a normal porcine kidney in 1983; cells have been used in assays to test toxins and mutagens
Reference(s) : 15163
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelial-like cells growing in monolayers; image; image
Medium : 80-90% Medium 199 (Hanks salts) + 10-20% h.i. FBS
Subculture : cells are difficult to culture and grow slowly to very slowly! split confluent culture 1:5 to 1:10 about once a week using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Harvest : cell harvest of about 8 x 106 cells/80 cm2 or 1 x 107 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization assays
Viruses : ELISA: reverse transcriptase positive; PCR: SMRV -
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AM-C6SC8

  • 货号: TS259067
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  • 品牌 : TESTOBIO
产品名称: AM-C6SC8
商品货号: TS259067
<strong>Cell line </strong>: AM-C6SC8
DSMZ no. : TS259067
Species : originally confirmed as pig by species PCR and recently as sus scrofa origin by COI DNA barcoding
Cell type : normal kidney cells
Origin : derived as a subclone from the cell line AM II which was established from a normal porcine kidney in 1983; cells have been used in assays to test toxins and mutagens
Reference(s) : 15163
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: epithelial-like cells growing in monolayers; image; image
Medium : 80-90% Medium 199 (Hanks salts) + 10-20% h.i. FBS
Subculture : cells are difficult to culture and grow slowly to very slowly! split confluent culture 1:5 to 1:10 about once a week using trypsin/EDTA
Incubation : at 37 °C with 5% CO2
Harvest : cell harvest of about 8 x 106 cells/80 cm2 or 1 x 107 cells/175 cm2
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization assays
Viruses : ELISA: reverse transcriptase positive; PCR: SMRV -
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