MHH-NB-11
MHH-NB-11
规格:
货期:
编号:TS259077
品牌:Testobio
| 产品名称： | MHH-NB-11 |
|---|---|
| 商品货号： | TS259077 |
| <strong>Cell line </strong>： | MHH-NB-11 |
| DSMZ no. ： | TS259077 |
| Species ： | confirmed originally as human with IEF of AST, MDH and later by STR typing |
| Cell type ： | neuroblastoma |
| Origin ： | established from a neuroblastoma metastasis at an adrenal site of a 4-year-old white boy in 1986; cells were described in the literature to express neuron-specific enolase and synaptophysin (but neither GFAP nor S-100 protein) and to carry a 20-fold amplification of MYCN (NMYC, N-myc) |
| Reference(s) ： | 14852 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, fibroblast-like cells growing as monolayers; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine + MEM non-essential amino acids |
| Subculture ： | seed out at 1.5-3 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 4-6 days using trypsin/EDTA; a significant number of cells may detach spontaneously from the plastic surface and these floating cells may also be used for passaging. Note: medium must be changed frequently to prevent acidity which induces cell clumping; optimal dissociation after trypsination is affected by gentle aspiration with a #21 gauge needle and syringe |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80-100 hours |
| Harvest ： | cell harvest of about 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human bimodal hyper-, diploid/tetraploid karyotype - 54(48-54)<2n>XY, +1, +1, +2, +6, +7, +8, +13, +20, der(1)t(1;?)(p32;?)x2, der(13)t(13;?2HSR)(q33;?2p23) - both the long HSR on ch 13 due to MYCN (NMYC, N-myc) amplification and the ch 1 short-arm rearrangements are typical of neuroblastoma - identity confirmed |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
MHH-NB-11
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | MHH-NB-11 |
|---|---|
| 商品货号： | TS259077 |
| <strong>Cell line </strong>： | MHH-NB-11 |
| DSMZ no. ： | TS259077 |
| Species ： | confirmed originally as human with IEF of AST, MDH and later by STR typing |
| Cell type ： | neuroblastoma |
| Origin ： | established from a neuroblastoma metastasis at an adrenal site of a 4-year-old white boy in 1986; cells were described in the literature to express neuron-specific enolase and synaptophysin (but neither GFAP nor S-100 protein) and to carry a 20-fold amplification of MYCN (NMYC, N-myc) |
| Reference(s) ： | 14852 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, fibroblast-like cells growing as monolayers; image; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine + MEM non-essential amino acids |
| Subculture ： | seed out at 1.5-3 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 4-6 days using trypsin/EDTA; a significant number of cells may detach spontaneously from the plastic surface and these floating cells may also be used for passaging. Note: medium must be changed frequently to prevent acidity which induces cell clumping; optimal dissociation after trypsination is affected by gentle aspiration with a #21 gauge needle and syringe |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 80-100 hours |
| Harvest ： | cell harvest of about 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human bimodal hyper-, diploid/tetraploid karyotype - 54(48-54)<2n>XY, +1, +1, +2, +6, +7, +8, +13, +20, der(1)t(1;?)(p32;?)x2, der(13)t(13;?2HSR)(q33;?2p23) - both the long HSR on ch 13 due to MYCN (NMYC, N-myc) amplification and the ch 1 short-arm rearrangements are typical of neuroblastoma - identity confirmed |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
