IMR-32
IMR-32
规格:
货期:
编号:TS259079
品牌:Testobio
| 产品名称： | IMR-32 |
|---|---|
| 商品货号： | TS259079 |
| <strong>Cell line </strong>： | IMR-32 |
| DSMZ no. ： | TS259079 |
| Species ： | confirmed originally as human with IEF of AST, MDH, PEP B and later by STR typing |
| Cell type ： | neuroblastoma |
| Origin ： | established from an abdominal mass (diagnosed as neuroblastoma) of a 1-year-old boy in 1967 |
| Reference(s) ： | 15025 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblast-like adherent cells growing as monolayer; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 1x non-essential amino acids |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-5 days (trypsin need not be used, cells can be detached by tapping the flask or pipetting) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | about 5-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 49(44-51)<2n>XY, +1, +6, +12, der(1)t(1qter->1p34::hsr::17q22->qter)x2, add(16)(q24) - sideline with del(3)(p14), add(7)(q35), del(9)(q34), add(19)(q13) - NB associated changes include: N-myc amplified in hsr regions and unbalanced t(1;17) - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | IMR-32 |
|---|---|
| 商品货号： | TS259079 |
| <strong>Cell line </strong>： | IMR-32 |
| DSMZ no. ： | TS259079 |
| Species ： | confirmed originally as human with IEF of AST, MDH, PEP B and later by STR typing |
| Cell type ： | neuroblastoma |
| Origin ： | established from an abdominal mass (diagnosed as neuroblastoma) of a 1-year-old boy in 1967 |
| Reference(s) ： | 15025 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | fibroblast-like adherent cells growing as monolayer; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 1x non-essential amino acids |
| Subculture ： | seed out at ca. 0.5-1.0 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-5 days (trypsin need not be used, cells can be detached by tapping the flask or pipetting) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | about 5-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 49(44-51)<2n>XY, +1, +6, +12, der(1)t(1qter->1p34::hsr::17q22->qter)x2, add(16)(q24) - sideline with del(3)(p14), add(7)(q35), del(9)(q34), add(19)(q13) - NB associated changes include: N-myc amplified in hsr regions and unbalanced t(1;17) - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
