MDBK
MDBK
规格:
货期:
编号:TS259102
品牌:Testobio
| 产品名称： | MDBK |
|---|---|
| 商品货号： | TS259102 |
| <strong>Cell line </strong>： | MDBK |
| DSMZ no. ： | TS259102 |
| Species ： | Bos taurus origin confirmed by COI DNA barcoding |
| Cell type ： | kidney cells |
| Origin ： | cells were derived from a kidney of a normal adult steer in 1957; cells are described to be BVD Virus-free (bovine diarrhea virus) |
| Reference(s) ： | 14729 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells; image; image |
| Medium ： | 95% Dulbeccos MEM + 5% h.i. FBS |
| Subculture ： | seed out 1-2 x 106 cells/80 cm2; optimal split ratio at 1:4 to 1:6 using trypsin/EDTA; cells grow within 2-3 days to confluence |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of about 5-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
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联系我们CONTACT US
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0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | MDBK |
|---|---|
| 商品货号： | TS259102 |
| <strong>Cell line </strong>： | MDBK |
| DSMZ no. ： | TS259102 |
| Species ： | Bos taurus origin confirmed by COI DNA barcoding |
| Cell type ： | kidney cells |
| Origin ： | cells were derived from a kidney of a normal adult steer in 1957; cells are described to be BVD Virus-free (bovine diarrhea virus) |
| Reference(s) ： | 14729 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells; image; image |
| Medium ： | 95% Dulbeccos MEM + 5% h.i. FBS |
| Subculture ： | seed out 1-2 x 106 cells/80 cm2; optimal split ratio at 1:4 to 1:6 using trypsin/EDTA; cells grow within 2-3 days to confluence |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35 hours |
| Harvest ： | cell harvest of about 5-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
