| 产品名称: | BTI-EAA |
|---|---|
| 商品货号: | TS259112 |
| <strong>Cell line </strong>: | BTI-EAA |
| DSMZ no. : | TS259112 |
| Species : | Estigmene acrea origin confirmed by COI DNA barcoding |
| Cell type : | |
| Origin : | established from the larvae of the Acrea Moth (Estigmene acrea; family Arctiidae, order Lepidoptera) (Baerenspinner) |
| Reference(s) : | not published |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | very large cells growing in suspension; most cells are round to oval, 10% have long processes and are spindle shaped |
| Medium : | 80% TC 100 medium + 20% h.i. FBS |
| Subculture : | cells are difficult to culture and grow slowly! split saturated culture 1:4 to 1:5 every 4-5 days |
| Incubation : | no CO2 (keep lid of flask closed) at room temperature (20-25 °C) |
| Harvest : | cell harvest of about 0.5 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| 产品名称: | BTI-EAA |
|---|---|
| 商品货号: | TS259112 |
| <strong>Cell line </strong>: | BTI-EAA |
| DSMZ no. : | TS259112 |
| Species : | Estigmene acrea origin confirmed by COI DNA barcoding |
| Cell type : | |
| Origin : | established from the larvae of the Acrea Moth (Estigmene acrea; family Arctiidae, order Lepidoptera) (Baerenspinner) |
| Reference(s) : | not published |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | very large cells growing in suspension; most cells are round to oval, 10% have long processes and are spindle shaped |
| Medium : | 80% TC 100 medium + 20% h.i. FBS |
| Subculture : | cells are difficult to culture and grow slowly! split saturated culture 1:4 to 1:5 every 4-5 days |
| Incubation : | no CO2 (keep lid of flask closed) at room temperature (20-25 °C) |
| Harvest : | cell harvest of about 0.5 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |