| 产品名称: | IPL-LD-65Y |
|---|---|
| 商品货号: | TS259121 |
| <strong>Cell line </strong>: | IPL-LD-65Y |
| DSMZ no. : | TS259121 |
| Species : | confirmed as not murine and not primate with IEF of AST, MDH, PEP B |
| Cell type : | larval cells |
| Origin : | established from the larvae of a gypsy moth (species Lymantria dispar, family Lymantriidae, order Lepidoptera) (Schwammspinner) |
| Reference(s) : | not published |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | large cells; up to 30% grow adherent with processes; suspension cells are round to oval; image; image |
| Medium : | 80% TC 100 medium + 20% h.i. FBS |
| Subculture : | cells are difficult to culture and grow slowly! split confluent culture 1:4 every 4-5 days using a (plastic) cell scraper |
| Incubation : | without exposure to CO2 (keep lid of flask closed) at room temperature (20-25 °C) |
| Harvest : | cell harvest of up to 10 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| 产品名称: | IPL-LD-65Y |
|---|---|
| 商品货号: | TS259121 |
| <strong>Cell line </strong>: | IPL-LD-65Y |
| DSMZ no. : | TS259121 |
| Species : | confirmed as not murine and not primate with IEF of AST, MDH, PEP B |
| Cell type : | larval cells |
| Origin : | established from the larvae of a gypsy moth (species Lymantria dispar, family Lymantriidae, order Lepidoptera) (Schwammspinner) |
| Reference(s) : | not published |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | large cells; up to 30% grow adherent with processes; suspension cells are round to oval; image; image |
| Medium : | 80% TC 100 medium + 20% h.i. FBS |
| Subculture : | cells are difficult to culture and grow slowly! split confluent culture 1:4 every 4-5 days using a (plastic) cell scraper |
| Incubation : | without exposure to CO2 (keep lid of flask closed) at room temperature (20-25 °C) |
| Harvest : | cell harvest of up to 10 x 106 cells/80 cm2 |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |