| 产品名称: | 2HX-2 |
|---|---|
| 商品货号: | TS259123 |
| <strong>Cell line </strong>: | 2HX-2 |
| DSMZ no. : | TS259123 |
| Species : | confirmed as mouse with IEF of AST, NP, PEP B and recently as mus musculus by COI DNA Barcoding |
| Cell type : | hybridoma (anti-adenovirus 2) |
| Origin : | established by fusion of SP2/0-AG14 myeloma cells with spleen cells from BALB/c mice immunized with HELA cells that were infected with adenovirus 2; cells secrete an IgG monoclonal antibody reacting with an adenovirus group specific antigen; cell line is patented |
| Reference(s) : | 14424 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | single cells growing in suspension |
| Medium : | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture : | optimal split ratio of 1:2 every 2-3 days |
| Incubation : | at 37 °C with 5% CO2 |
| Harvest : | cell harvest of about 1-2 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics : | murine karyotype with centric fusion and 16% polyploidy - 66(58-74)<3n> |
| Viruses : | ELISA: reverse transcriptase negative; PCR: SMRV - |
| 产品名称: | 2HX-2 |
|---|---|
| 商品货号: | TS259123 |
| <strong>Cell line </strong>: | 2HX-2 |
| DSMZ no. : | TS259123 |
| Species : | confirmed as mouse with IEF of AST, NP, PEP B and recently as mus musculus by COI DNA Barcoding |
| Cell type : | hybridoma (anti-adenovirus 2) |
| Origin : | established by fusion of SP2/0-AG14 myeloma cells with spleen cells from BALB/c mice immunized with HELA cells that were infected with adenovirus 2; cells secrete an IgG monoclonal antibody reacting with an adenovirus group specific antigen; cell line is patented |
| Reference(s) : | 14424 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | single cells growing in suspension |
| Medium : | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture : | optimal split ratio of 1:2 every 2-3 days |
| Incubation : | at 37 °C with 5% CO2 |
| Harvest : | cell harvest of about 1-2 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics : | murine karyotype with centric fusion and 16% polyploidy - 66(58-74)<3n> |
| Viruses : | ELISA: reverse transcriptase negative; PCR: SMRV - |