LCL-HO
LCL-HO
规格:
货期:
编号:TS259125
品牌:Testobio
| 产品名称： | LCL-HO |
|---|---|
| 商品货号： | TS259125 |
| <strong>Cell line </strong>： | LCL-HO |
| DSMZ no. ： | TS259125 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | B lymphoblastoid cells (from patient with melanoma) |
| Origin ： | this lymphoblastoid cell line was established by EBV transformation of peripheral blood lymphocytes; cell line was submitted as autologous to cell line MEL-HO (DSM ACC 62) that was derived from a woman with primary melanoma in 1976; however, cytogenetic and DNA fingerprinting analysis showed clearly that LCL-HO and MEL-HO are not related |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A, C |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid-like cells growing in suspension singly or in clumps |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | saturation density at about 2.0-2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cy IgM -, cykappa -, cylambda +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human diploid karyotype with 2% polyploidy and near triploid sideline (4%) - 46(45-47)<2n>XX, der(12)t(12;?6)(q24.32;p21.2) - additional pseudodiploid subclones are present with other structural and numerical rearrangements |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV + |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | LCL-HO |
|---|---|
| 商品货号： | TS259125 |
| <strong>Cell line </strong>： | LCL-HO |
| DSMZ no. ： | TS259125 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | B lymphoblastoid cells (from patient with melanoma) |
| Origin ： | this lymphoblastoid cell line was established by EBV transformation of peripheral blood lymphocytes; cell line was submitted as autologous to cell line MEL-HO (DSM ACC 62) that was derived from a woman with primary melanoma in 1976; however, cytogenetic and DNA fingerprinting analysis showed clearly that LCL-HO and MEL-HO are not related |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A, C |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | lymphoblastoid-like cells growing in suspension singly or in clumps |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | maintain at 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-4 days; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 30 hours |
| Harvest ： | saturation density at about 2.0-2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cy IgM -, cykappa -, cylambda +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human diploid karyotype with 2% polyploidy and near triploid sideline (4%) - 46(45-47)<2n>XX, der(12)t(12;?6)(q24.32;p21.2) - additional pseudodiploid subclones are present with other structural and numerical rearrangements |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV + |
