| 产品名称: | SPC-BM-36 |
|---|---|
| 商品货号: | TS259126 |
| <strong>Cell line </strong>: | SPC-BM-36 |
| DSMZ no. : | TS259126 |
| Species : | Spodoptera frugiperda origin determined by COI DNA barcoding |
| Cell type : | larval cells |
| Origin : | derived from larvae of a silkworm (species Bombyx mori, family Bombycidae, order Lepidoptera); however, COI DNA barcoding revealed SPC-BM-36 as originating from a fall armyworm (Spodoptera frugiperda) and not from a silkworm as described |
| Reference(s) : | 14, 17 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | large, mostly adherent cells; 90% round cells (singly or aggregates), 10% spindled-shaped cells with long processes |
| Medium : | 80% TC 100 medium + 20% h.i. FBS |
| Subculture : | cells are difficult to culture and grow slowly! split confluent culture 1:3 to 1:6 every 3-4 days using a (plastic) cell scraper |
| Incubation : | cells grow without CO2 (keep lid of flask closed) at room temperature (20-25 °C) |
| Doubling time : | ca. 50-100 hours |
| Harvest : | cell harvest of up to 12 x 106 cells/80 cm2 flask |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| 产品名称: | SPC-BM-36 |
|---|---|
| 商品货号: | TS259126 |
| <strong>Cell line </strong>: | SPC-BM-36 |
| DSMZ no. : | TS259126 |
| Species : | Spodoptera frugiperda origin determined by COI DNA barcoding |
| Cell type : | larval cells |
| Origin : | derived from larvae of a silkworm (species Bombyx mori, family Bombycidae, order Lepidoptera); however, COI DNA barcoding revealed SPC-BM-36 as originating from a fall armyworm (Spodoptera frugiperda) and not from a silkworm as described |
| Reference(s) : | 14, 17 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | large, mostly adherent cells; 90% round cells (singly or aggregates), 10% spindled-shaped cells with long processes |
| Medium : | 80% TC 100 medium + 20% h.i. FBS |
| Subculture : | cells are difficult to culture and grow slowly! split confluent culture 1:3 to 1:6 every 3-4 days using a (plastic) cell scraper |
| Incubation : | cells grow without CO2 (keep lid of flask closed) at room temperature (20-25 °C) |
| Doubling time : | ca. 50-100 hours |
| Harvest : | cell harvest of up to 12 x 106 cells/80 cm2 flask |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |