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SPC-BM-36
SPC-BM-36
规格:
货期:
编号:TS259126
品牌:Testobio
产品名称: SPC-BM-36
商品货号: TS259126
<strong>Cell line </strong>: SPC-BM-36
DSMZ no. : TS259126
Species : Spodoptera frugiperda origin determined by COI DNA barcoding
Cell type : larval cells
Origin : derived from larvae of a silkworm (species Bombyx mori, family Bombycidae, order Lepidoptera); however, COI DNA barcoding revealed SPC-BM-36 as originating from a fall armyworm (Spodoptera frugiperda) and not from a silkworm as described
Reference(s) : 14, 17
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: large, mostly adherent cells; 90% round cells (singly or aggregates), 10% spindled-shaped cells with long processes
Medium : 80% TC 100 medium + 20% h.i. FBS
Subculture : cells are difficult to culture and grow slowly! split confluent culture 1:3 to 1:6 every 3-4 days using a (plastic) cell scraper
Incubation : cells grow without CO2 (keep lid of flask closed) at room temperature (20-25 °C)
Doubling time : ca. 50-100 hours
Harvest : cell harvest of up to 12 x 106 cells/80 cm2 flask
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization assays
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SPC-BM-36

  • 货号: TS259126
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  • 品牌 : TESTOBIO
产品名称: SPC-BM-36
商品货号: TS259126
<strong>Cell line </strong>: SPC-BM-36
DSMZ no. : TS259126
Species : Spodoptera frugiperda origin determined by COI DNA barcoding
Cell type : larval cells
Origin : derived from larvae of a silkworm (species Bombyx mori, family Bombycidae, order Lepidoptera); however, COI DNA barcoding revealed SPC-BM-36 as originating from a fall armyworm (Spodoptera frugiperda) and not from a silkworm as described
Reference(s) : 14, 17
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: large, mostly adherent cells; 90% round cells (singly or aggregates), 10% spindled-shaped cells with long processes
Medium : 80% TC 100 medium + 20% h.i. FBS
Subculture : cells are difficult to culture and grow slowly! split confluent culture 1:3 to 1:6 every 3-4 days using a (plastic) cell scraper
Incubation : cells grow without CO2 (keep lid of flask closed) at room temperature (20-25 °C)
Doubling time : ca. 50-100 hours
Harvest : cell harvest of up to 12 x 106 cells/80 cm2 flask
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization assays
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