COLO-800
COLO-800
规格:
货期:
编号:TS259142
品牌:Testobio
| 产品名称： | COLO-800 |
|---|---|
| 商品货号： | TS259142 |
| <strong>Cell line </strong>： | COLO-800 |
| DSMZ no. ： | TS259142 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | melanoma |
| Origin ： | established from the subcutaneous nodule of a 14-year-old boy with melanoma in 1990 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing as monolayer; some cells look like fibroblasts or neurons |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | split confluent culture 1:4 to 1:5 two to three times per week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours (with 5% FBS) |
| Harvest ： | cell harvest of about 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with minor hypertriploid clone and 8% polyploidy - 64(50-69)<3n>X, -Y, -X/Y, -4, -5, +7, -8, -9, -10, -11, -12, +13, +13, +14, +15, -16, -19, +20, -21, -21, der(3)t(3;?)(p11;?), der(13q21q)x2, del(14)(?q11-12q24.2)x2, der(15)t(15;?)(q26;?)x2 - identity confirmed by marker correspondence |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | COLO-800 |
|---|---|
| 商品货号： | TS259142 |
| <strong>Cell line </strong>： | COLO-800 |
| DSMZ no. ： | TS259142 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | melanoma |
| Origin ： | established from the subcutaneous nodule of a 14-year-old boy with melanoma in 1990 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing as monolayer; some cells look like fibroblasts or neurons |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | split confluent culture 1:4 to 1:5 two to three times per week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours (with 5% FBS) |
| Harvest ： | cell harvest of about 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with minor hypertriploid clone and 8% polyploidy - 64(50-69)<3n>X, -Y, -X/Y, -4, -5, +7, -8, -9, -10, -11, -12, +13, +13, +14, +15, -16, -19, +20, -21, -21, der(3)t(3;?)(p11;?), der(13q21q)x2, del(14)(?q11-12q24.2)x2, der(15)t(15;?)(q26;?)x2 - identity confirmed by marker correspondence |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
