23132/87
23132/87
规格:
货期:
编号:TS259167
品牌:Testobio
| 产品名称： | 23132/87 |
|---|---|
| 商品货号： | TS259167 |
| <strong>Cell line </strong>： | 23132/87 |
| DSMZ no. ： | TS259167 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | gastric adenocarcinoma |
| Origin ： | established from the primary tumor of a 72-year-old man with gastric adenocarcinoma in 1987 |
| Reference(s) ： | 15047 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial cells growing as confluent monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out with 1-2 x 106/25 cm2 flask; split confluent culture 1:8 every 5-6 days using trypsin/EDTA; initially after thawing, the viability might decrease down to ca. 20-50% during the first week and cells may grow slowly and loosely adherent, but cells recover subsequently over the next week and viability will raise over the next few passages |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50 hours |
| Harvest ： | cell harvest of 10-12 x 106 cells/80cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertetraploid karyotype with 12% polyploidy - 47(45-52)<2n>XY, +20, t(1;15)(p11;p11), t(6;12) (p21;q21), i(13q), t(13;14)(p10;q10) - closely resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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13372109301
| 产品名称： | 23132/87 |
|---|---|
| 商品货号： | TS259167 |
| <strong>Cell line </strong>： | 23132/87 |
| DSMZ no. ： | TS259167 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | gastric adenocarcinoma |
| Origin ： | established from the primary tumor of a 72-year-old man with gastric adenocarcinoma in 1987 |
| Reference(s) ： | 15047 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial cells growing as confluent monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out with 1-2 x 106/25 cm2 flask; split confluent culture 1:8 every 5-6 days using trypsin/EDTA; initially after thawing, the viability might decrease down to ca. 20-50% during the first week and cells may grow slowly and loosely adherent, but cells recover subsequently over the next week and viability will raise over the next few passages |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50 hours |
| Harvest ： | cell harvest of 10-12 x 106 cells/80cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertetraploid karyotype with 12% polyploidy - 47(45-52)<2n>XY, +20, t(1;15)(p11;p11), t(6;12) (p21;q21), i(13q), t(13;14)(p10;q10) - closely resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
