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A-S-30D
A-S-30D
规格:
货期:
编号:TS259177
品牌:Testobio
产品名称: A-S-30D
商品货号: TS259177
<strong>Cell line </strong>: A-S-30D
DSMZ no. : TS259177
Species : originally confirmed as rat with IEF and by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding
Cell type : hepatoma
Origin : established from the hepatoma of a 16-month-old SD-female rat in 1988
Reference(s) : 14433, 15140, 15202
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: grows as single cells in suspension
Medium : 90% Dulbeccos MEM + 10% h.i. FBS
Subculture : optimal split ratio of 1:2 to 1:3 twice per week
Incubation : at 37 °C with 5% CO2
Doubling time : about 29 hours
Harvest : cell harvest of about 0.6 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization assays
Fingerprint : unique DNA profile with (gtg)5 multilocus probe
Cytogenetics : hypodiploid rat karyotype with 12% tetraploidy - 38(35-41)<2n> - 2 large submetacentric and 1 large metacentric centric fusion markers - sideline with 30-32/60-62 present at 14%
Viruses : ELISA: reverse transcriptase negative; PCR: SMRV -
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A-S-30D

  • 货号: TS259177
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  • 品牌 : TESTOBIO
产品名称: A-S-30D
商品货号: TS259177
<strong>Cell line </strong>: A-S-30D
DSMZ no. : TS259177
Species : originally confirmed as rat with IEF and by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding
Cell type : hepatoma
Origin : established from the hepatoma of a 16-month-old SD-female rat in 1988
Reference(s) : 14433, 15140, 15202
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: grows as single cells in suspension
Medium : 90% Dulbeccos MEM + 10% h.i. FBS
Subculture : optimal split ratio of 1:2 to 1:3 twice per week
Incubation : at 37 °C with 5% CO2
Doubling time : about 29 hours
Harvest : cell harvest of about 0.6 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization assays
Fingerprint : unique DNA profile with (gtg)5 multilocus probe
Cytogenetics : hypodiploid rat karyotype with 12% tetraploidy - 38(35-41)<2n> - 2 large submetacentric and 1 large metacentric centric fusion markers - sideline with 30-32/60-62 present at 14%
Viruses : ELISA: reverse transcriptase negative; PCR: SMRV -
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