| 产品名称: | A-S-30D |
|---|---|
| 商品货号: | TS259177 |
| <strong>Cell line </strong>: | A-S-30D |
| DSMZ no. : | TS259177 |
| Species : | originally confirmed as rat with IEF and by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type : | hepatoma |
| Origin : | established from the hepatoma of a 16-month-old SD-female rat in 1988 |
| Reference(s) : | 14433, 15140, 15202 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | grows as single cells in suspension |
| Medium : | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture : | optimal split ratio of 1:2 to 1:3 twice per week |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | about 29 hours |
| Harvest : | cell harvest of about 0.6 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Fingerprint : | unique DNA profile with (gtg)5 multilocus probe |
| Cytogenetics : | hypodiploid rat karyotype with 12% tetraploidy - 38(35-41)<2n> - 2 large submetacentric and 1 large metacentric centric fusion markers - sideline with 30-32/60-62 present at 14% |
| Viruses : | ELISA: reverse transcriptase negative; PCR: SMRV - |
| 产品名称: | A-S-30D |
|---|---|
| 商品货号: | TS259177 |
| <strong>Cell line </strong>: | A-S-30D |
| DSMZ no. : | TS259177 |
| Species : | originally confirmed as rat with IEF and by species PCR and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type : | hepatoma |
| Origin : | established from the hepatoma of a 16-month-old SD-female rat in 1988 |
| Reference(s) : | 14433, 15140, 15202 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | grows as single cells in suspension |
| Medium : | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture : | optimal split ratio of 1:2 to 1:3 twice per week |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | about 29 hours |
| Harvest : | cell harvest of about 0.6 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization assays |
| Fingerprint : | unique DNA profile with (gtg)5 multilocus probe |
| Cytogenetics : | hypodiploid rat karyotype with 12% tetraploidy - 38(35-41)<2n> - 2 large submetacentric and 1 large metacentric centric fusion markers - sideline with 30-32/60-62 present at 14% |
| Viruses : | ELISA: reverse transcriptase negative; PCR: SMRV - |