A4-951
A4-951
规格:
货期:
编号:TS259185
品牌:Testobio
| 产品名称： | A4-951 |
|---|---|
| 商品货号： | TS259185 |
| <strong>Cell line </strong>： | A4-951 |
| DSMZ no. ： | TS259185 |
| Species ： | originally confirmed as mouse with IEF of AST, NP and with species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) reacting with adult slow fibers in rat and human and with slow myosin heavy chain on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin |
| Reference(s) ： | 14620 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells, lightly adherent, growing singly and in clumps |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids |
| Subculture ： | split saturated culture 1:2 to 1:3 every 2-3 days; seed out at ca. 0.2 x 106 cells/ml; optimal density of about 0.2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 24 hours |
| Harvest ： | maximal density of about 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Cytogenetics ： | murine flat-moded hypertetraploid karyotype - 81(42-92)<4n> - 1-2 dmin and 1-2 centric fusion markers present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
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周雪
13372109301
| 产品名称： | A4-951 |
|---|---|
| 商品货号： | TS259185 |
| <strong>Cell line </strong>： | A4-951 |
| DSMZ no. ： | TS259185 |
| Species ： | originally confirmed as mouse with IEF of AST, NP and with species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) reacting with adult slow fibers in rat and human and with slow myosin heavy chain on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin |
| Reference(s) ： | 14620 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells, lightly adherent, growing singly and in clumps |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids |
| Subculture ： | split saturated culture 1:2 to 1:3 every 2-3 days; seed out at ca. 0.2 x 106 cells/ml; optimal density of about 0.2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 24 hours |
| Harvest ： | maximal density of about 0.8 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Cytogenetics ： | murine flat-moded hypertetraploid karyotype - 81(42-92)<4n> - 1-2 dmin and 1-2 centric fusion markers present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
