CRO-AP5
CRO-AP5
规格:
货期:
编号:TS259187
品牌:Testobio
| 产品名称： | CRO-AP5 |
|---|---|
| 商品货号： | TS259187 |
| <strong>Cell line </strong>： | CRO-AP5 |
| DSMZ no. ： | TS259187 |
| Species ： | confirmed as human with IEF of AST, LDH |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1998 from the pleural effusion of a 35-year-old HIV-positive homosexual man with primary effusion lymphoma (PEL) and a history of Kaposis sarcoma; cells are HHV-8-positive, but HIV-negative. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 15032, 14418, 14483, 18187 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round to pleomorph cells growing in suspension |
| Medium ： | 80-85% RPMI 1640 + 15-20% h.i. FBS |
| Subculture ： | split saturated culture 1:3 every 3-4 days; maintain at ca. 0.5-1.0 x 106 cells/ml; seed out at ca. 1.0 x 106 cells/ml in 24- or 12-well plates; viability may be low after thawing and during the first 1-2 weeks |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-40 hours |
| Harvest ： | maximal density at ca. 1.4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD37 +, cyCD79a -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, cm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 12% polyploidy - 44-49<2n>X, -Y, +8, +2mar, del(4)(q25), add(8)(p11), dup(12)(q11q14), del(14)(q24) - sideline with i(8q) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | CRO-AP5 |
|---|---|
| 商品货号： | TS259187 |
| <strong>Cell line </strong>： | CRO-AP5 |
| DSMZ no. ： | TS259187 |
| Species ： | confirmed as human with IEF of AST, LDH |
| Cell type ： | B cell lymphoma |
| Origin ： | established in 1998 from the pleural effusion of a 35-year-old HIV-positive homosexual man with primary effusion lymphoma (PEL) and a history of Kaposis sarcoma; cells are HHV-8-positive, but HIV-negative. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 15032, 14418, 14483, 18187 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round to pleomorph cells growing in suspension |
| Medium ： | 80-85% RPMI 1640 + 15-20% h.i. FBS |
| Subculture ： | split saturated culture 1:3 every 3-4 days; maintain at ca. 0.5-1.0 x 106 cells/ml; seed out at ca. 1.0 x 106 cells/ml in 24- or 12-well plates; viability may be low after thawing and during the first 1-2 weeks |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 35-40 hours |
| Harvest ： | maximal density at ca. 1.4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD10 -, CD13 -, CD19 -, CD34 -, CD37 +, cyCD79a -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, cm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 12% polyploidy - 44-49<2n>X, -Y, +8, +2mar, del(4)(q25), add(8)(p11), dup(12)(q11q14), del(14)(q24) - sideline with i(8q) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
