8505C
8505C
规格:
货期:
编号:TS259192
品牌:Testobio
| 产品名称： | 8505C |
|---|---|
| 商品货号： | TS259192 |
| <strong>Cell line </strong>： | 8505C |
| DSMZ no. ： | TS259192 |
| Species ： | confirmed originally as human with IEF of AST, LDH, NP and later by STR-fingerprinting |
| Cell type ： | thyroid carcinoma |
| Origin ： | established from the primary tumor of a 78-year-old woman with thyroid carcinoma (undifferentiated carcinoma, histologically a largely papillary adenocarcinoma with some spindle, polygonal and giant cells) |
| Reference(s) ： | 14631 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing as monolayer; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:5 every 2-3 days or 1:10 every 4-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-40 hours |
| Harvest ： | cell harvest of about 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 16% polyploidy - 65(57-68)<3n>XX, -X, -2, +3, -7, -10, +11, -13, -16, -18, -19, +2mar, del(1)(q13), del(3)(q22), ?del(4)(q21), i(8q) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | 8505C |
|---|---|
| 商品货号： | TS259192 |
| <strong>Cell line </strong>： | 8505C |
| DSMZ no. ： | TS259192 |
| Species ： | confirmed originally as human with IEF of AST, LDH, NP and later by STR-fingerprinting |
| Cell type ： | thyroid carcinoma |
| Origin ： | established from the primary tumor of a 78-year-old woman with thyroid carcinoma (undifferentiated carcinoma, histologically a largely papillary adenocarcinoma with some spindle, polygonal and giant cells) |
| Reference(s) ： | 14631 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing as monolayer; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:5 every 2-3 days or 1:10 every 4-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-40 hours |
| Harvest ： | cell harvest of about 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 16% polyploidy - 65(57-68)<3n>XX, -X, -2, +3, -7, -10, +11, -13, -16, -18, -19, +2mar, del(1)(q13), del(3)(q22), ?del(4)(q21), i(8q) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
