G/G
G/G
规格:
货期:
编号:TS259201
品牌:Testobio
| 产品名称： | G/G |
|---|---|
| 商品货号： | TS259201 |
| <strong>Cell line </strong>： | G/G |
| DSMZ no. ： | TS259201 |
| Species ： | confirmed as mouse with IEF of AST, NP and by COI DNA barcoding species PCR; strain analysis indeed suggests a C3H-substrain |
| Cell type ： | urinary bladder |
| Origin ： | established from an adult female C3H/E1 mouse; non-tumorigenic urine bladder cell line immortalized in vitro |
| Reference(s) ： | 14449 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial, adherent cells, growing in up to 3 layers; image; image |
| Medium ： | 45% Hams F10 + 45% Dulbeccos MEM + 10% h.i. FBS + 5 µg/ml human insulin + 5 µg/ml human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 every 3-7 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 35-50 hours |
| Harvest ： | harvest of about 0.5 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near-tetraploid karyotype with 3.6% polyploidy - 80/81(76-83)<4n> - up to 3 (sub)metacentric markers present in most cells arising by centric fusion |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
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章丽敏
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朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | G/G |
|---|---|
| 商品货号： | TS259201 |
| <strong>Cell line </strong>： | G/G |
| DSMZ no. ： | TS259201 |
| Species ： | confirmed as mouse with IEF of AST, NP and by COI DNA barcoding species PCR; strain analysis indeed suggests a C3H-substrain |
| Cell type ： | urinary bladder |
| Origin ： | established from an adult female C3H/E1 mouse; non-tumorigenic urine bladder cell line immortalized in vitro |
| Reference(s) ： | 14449 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial, adherent cells, growing in up to 3 layers; image; image |
| Medium ： | 45% Hams F10 + 45% Dulbeccos MEM + 10% h.i. FBS + 5 µg/ml human insulin + 5 µg/ml human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:10 every 3-7 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 35-50 hours |
| Harvest ： | harvest of about 0.5 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near-tetraploid karyotype with 3.6% polyploidy - 80/81(76-83)<4n> - up to 3 (sub)metacentric markers present in most cells arising by centric fusion |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
