BF-45
BF-45
规格:
货期:
编号:TS259207
品牌:Testobio
| 产品名称： | BF-45 |
|---|---|
| 商品货号： | TS259207 |
| <strong>Cell line </strong>： | BF-45 |
| DSMZ no. ： | TS259207 |
| Species ： | originally confirmed as mouse with IEF of NP, PEP B and by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) that reacts with rat embryonic fibers in immunofluorescence and with embryonic myosin heavy chains in Western blotting; established by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles |
| Reference(s) ： | 14928 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells) |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 0.25 x 106 cells/ml; split saturated culture at 1:3 to 1:4 every 2 days; viability may be low after thawing, but cells will recover soon |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 45 hours |
| Harvest ： | maximal density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Cytogenetics ： | murine flat-moded hypertetraploid karyotype with 5% polyploidy - 78-93<4n> - 1-2 dmin present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
BF-45
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | BF-45 |
|---|---|
| 商品货号： | TS259207 |
| <strong>Cell line </strong>： | BF-45 |
| DSMZ no. ： | TS259207 |
| Species ： | originally confirmed as mouse with IEF of NP, PEP B and by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) that reacts with rat embryonic fibers in immunofluorescence and with embryonic myosin heavy chains in Western blotting; established by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles |
| Reference(s) ： | 14928 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells) |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 0.25 x 106 cells/ml; split saturated culture at 1:3 to 1:4 every 2 days; viability may be low after thawing, but cells will recover soon |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 45 hours |
| Harvest ： | maximal density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Cytogenetics ： | murine flat-moded hypertetraploid karyotype with 5% polyploidy - 78-93<4n> - 1-2 dmin present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
