SC-75
SC-75
规格:
货期:
编号:TS259219
品牌:Testobio
| 产品名称： | SC-75 |
|---|---|
| 商品货号： | TS259219 |
| <strong>Cell line </strong>： | SC-75 |
| DSMZ no. ： | TS259219 |
| Species ： | originally confirmed as mouse with IEF of AST, NP and PEP B, by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgM) reacting with all adult rat type 2 fibers in immunofluorescence and with all adult rat type 2 MHCs (myosin heavy chain) in Western blotting; recognizing also rat neonatal fibers in immunofluorescence and rat neonatal MHCs in Western blotting; established in 1985 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine subcutaneous muscle |
| Reference(s) ： | 14929 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; some cells are loosely adherent (detachable by shaking the flask) |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1 week post-thawing |
| Subculture ： | maintain at 0.5-1.0 x 106 cells/ml; split saturated culture at 1:2 to 1:3 every 2 day (maximal split ratio of 1:5); viability may drop below 50% after thawing; we recommend to start culture with a cell concentration of ca. 0.5 x 106 cells/ml and to cultivate initially in 24-well plates with medium containing 20% horse serum |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 23 hours |
| Harvest ： | maximal density at about 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgM monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid karyotype with 5% polyploidy - 86-96<4n> - 1-3 centric fusion markers and 1-2 dmin present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
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章丽敏
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朱静
18067106081 -
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李晶晶
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周雪
13372109301
| 产品名称： | SC-75 |
|---|---|
| 商品货号： | TS259219 |
| <strong>Cell line </strong>： | SC-75 |
| DSMZ no. ： | TS259219 |
| Species ： | originally confirmed as mouse with IEF of AST, NP and PEP B, by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgM) reacting with all adult rat type 2 fibers in immunofluorescence and with all adult rat type 2 MHCs (myosin heavy chain) in Western blotting; recognizing also rat neonatal fibers in immunofluorescence and rat neonatal MHCs in Western blotting; established in 1985 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine subcutaneous muscle |
| Reference(s) ： | 14929 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells in suspension; some cells are loosely adherent (detachable by shaking the flask) |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1 week post-thawing |
| Subculture ： | maintain at 0.5-1.0 x 106 cells/ml; split saturated culture at 1:2 to 1:3 every 2 day (maximal split ratio of 1:5); viability may drop below 50% after thawing; we recommend to start culture with a cell concentration of ca. 0.5 x 106 cells/ml and to cultivate initially in 24-well plates with medium containing 20% horse serum |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 23 hours |
| Harvest ： | maximal density at about 2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgM monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid karyotype with 5% polyploidy - 86-96<4n> - 1-3 centric fusion markers and 1-2 dmin present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
