EFE-184
EFE-184
规格:
货期:
编号:TS259226
品牌:Testobio
| 产品名称： | EFE-184 |
|---|---|
| 商品货号： | TS259226 |
| <strong>Cell line </strong>： | EFE-184 |
| DSMZ no. ： | TS259226 |
| Species ： | originally confirmed as human with IEF of AST, NP and recently by STR fingerprinting |
| Cell type ： | endometrium carcinoma |
| Origin ： | established from the ascitic fluid of a 69-year-old Caucasian woman with endometrial carcinoma in relapse in 1985 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out about 1 x 106/175 cm2; split confluent culture 1:4 once a week using trypsin/EDTA; cells grow very slowly |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 90 hours |
| Harvest ： | cell harvest of about 4-5 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 1-2 supernumerary dm in most cells and 23% polyploidy - 80(72-81)<3n>XX, -X, +1, +2, -4, -5, +6, +8, +10, -13, +17, +19, +20, +21, +6mar - der(1)t(1;?)(p11;?)x2, del(2)(q24)x2, der(2)t(2;3)(q11;p11), del(12)(p13), der(16)t(16;?)(q21/23;?), der(17)t(7;17)(q11;p11), add(22)(p11)x2 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | EFE-184 |
|---|---|
| 商品货号： | TS259226 |
| <strong>Cell line </strong>： | EFE-184 |
| DSMZ no. ： | TS259226 |
| Species ： | originally confirmed as human with IEF of AST, NP and recently by STR fingerprinting |
| Cell type ： | endometrium carcinoma |
| Origin ： | established from the ascitic fluid of a 69-year-old Caucasian woman with endometrial carcinoma in relapse in 1985 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out about 1 x 106/175 cm2; split confluent culture 1:4 once a week using trypsin/EDTA; cells grow very slowly |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 90 hours |
| Harvest ： | cell harvest of about 4-5 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 1-2 supernumerary dm in most cells and 23% polyploidy - 80(72-81)<3n>XX, -X, +1, +2, -4, -5, +6, +8, +10, -13, +17, +19, +20, +21, +6mar - der(1)t(1;?)(p11;?)x2, del(2)(q24)x2, der(2)t(2;3)(q11;p11), del(12)(p13), der(16)t(16;?)(q21/23;?), der(17)t(7;17)(q11;p11), add(22)(p11)x2 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
