EFO-21
EFO-21
规格:
货期:
编号:TS259231
品牌:Testobio
| 产品名称： | EFO-21 |
|---|---|
| 商品货号： | TS259231 |
| <strong>Cell line </strong>： | EFO-21 |
| DSMZ no. ： | TS259231 |
| Species ： | originally confirmed as human with IEF of AST and MDH and later by STR fingerprinting |
| Cell type ： | ovary cystadenocarcinoma |
| Origin ： | established from the ascitic fluid of a 56-year-old Caucasian woman with dedifferentiated serous cystadenocarcinoma of the ovary in 1979 |
| Reference(s) ： | 14961 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid adherent cells growing in monolayers; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine + 1x MEM non-essential amino acids + 1 mM sodium pyruvate |
| Subculture ： | seed out about 1-2 x 106 cells/175 cm2; split confluent culture once a week using trypsin/EDTA; after thawing cells might grow very slowly |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50-60 hours |
| Harvest ： | cell harvest of about 6 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | apparently human karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
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朱静
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李晴
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李晶晶
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周雪
13372109301
| 产品名称： | EFO-21 |
|---|---|
| 商品货号： | TS259231 |
| <strong>Cell line </strong>： | EFO-21 |
| DSMZ no. ： | TS259231 |
| Species ： | originally confirmed as human with IEF of AST and MDH and later by STR fingerprinting |
| Cell type ： | ovary cystadenocarcinoma |
| Origin ： | established from the ascitic fluid of a 56-year-old Caucasian woman with dedifferentiated serous cystadenocarcinoma of the ovary in 1979 |
| Reference(s) ： | 14961 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid adherent cells growing in monolayers; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine + 1x MEM non-essential amino acids + 1 mM sodium pyruvate |
| Subculture ： | seed out about 1-2 x 106 cells/175 cm2; split confluent culture once a week using trypsin/EDTA; after thawing cells might grow very slowly |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50-60 hours |
| Harvest ： | cell harvest of about 6 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | apparently human karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
