IPC-298
IPC-298
规格:
货期:
编号:TS259261
品牌:Testobio
| 产品名称： | IPC-298 |
|---|---|
| 商品货号： | TS259261 |
| <strong>Cell line </strong>： | IPC-298 |
| DSMZ no. ： | TS259261 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | melanoma |
| Origin ： | established from the primary tumor (right cervical) of a 64-year-old woman with cutaneous melanoma |
| Reference(s) ： | 14364 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, spindle-like cells (a few epitheliod cells) growing as confluent monolayer; image |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | split confluent culture 1:8 to 1:10 every 4-5 days using trypsin/EDTA; seed out at ca. 3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30 hours |
| Harvest ： | cell harvest of about 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 13% polyploidy - 74(74-88)<3n>XX, -X, +1, -2, +3, -4, +6, +7, +9, -11, -11, +12, -13, -14, -15, +16, +21, +21, +4mar, dmin in 34% cells, del(3)(p14)x2, del(3)(q26.3)x2, del(6)(p23), del(7)(p14), add(7)(p22;HSR), der(9)t(9;11)(p21;q11), add(12)(p13)x3, del(12)(p13), del(17)(p12)x1-2 - sidelines with additional numerical and structural ch 7 rearrangements |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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IPC-298
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0574-87917803
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testobio@163.com
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | IPC-298 |
|---|---|
| 商品货号： | TS259261 |
| <strong>Cell line </strong>： | IPC-298 |
| DSMZ no. ： | TS259261 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | melanoma |
| Origin ： | established from the primary tumor (right cervical) of a 64-year-old woman with cutaneous melanoma |
| Reference(s) ： | 14364 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, spindle-like cells (a few epitheliod cells) growing as confluent monolayer; image |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | split confluent culture 1:8 to 1:10 every 4-5 days using trypsin/EDTA; seed out at ca. 3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30 hours |
| Harvest ： | cell harvest of about 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 13% polyploidy - 74(74-88)<3n>XX, -X, +1, -2, +3, -4, +6, +7, +9, -11, -11, +12, -13, -14, -15, +16, +21, +21, +4mar, dmin in 34% cells, del(3)(p14)x2, del(3)(q26.3)x2, del(6)(p23), del(7)(p14), add(7)(p22;HSR), der(9)t(9;11)(p21;q11), add(12)(p13)x3, del(12)(p13), del(17)(p12)x1-2 - sidelines with additional numerical and structural ch 7 rearrangements |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
