EFM-192A
EFM-192A
规格:
货期:
编号:TS259274
品牌:Testobio
| 产品名称： | EFM-192A |
|---|---|
| 商品货号： | TS259274 |
| <strong>Cell line </strong>： | EFM-192A |
| DSMZ no. ： | TS259274 |
| Species ： | originally confirmed as human with IEF of AST, NP, MDH, later by STR typing |
| Cell type ： | breast carcinoma |
| Origin ： | established from the pleural effusion of the left side from a 46-year-old Caucasian woman with metastasizing breast adenocarcinoma in 1985; sister cell lines EFM-192B and C show differences in molecular aspects; erbB-2 gene is amplified and overexpressed |
| Reference(s) ： | 15156 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid, adherent growing as monolayer; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:2 to 1:4 once a week using trypsin/EDTA; seed out at ca. 2 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 5-8 days |
| Harvest ： | cell harvest of about 8-12 x 106 cells/ 80cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as EFM-192B/EFM-192C using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human flat-moded near-triploid karyotype with 20% polyploidy - 68(60-70)<3n>XXXX, +X, -1, -4, +9, -12, -17, -17, -18, -22, +6mar, add(1)(p22), add(1)(p?32), add(2)(q35/37), add(3)(q27/29), i(15q), add(17)(p12), dminx1 present at 38% |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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EFM-192A
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | EFM-192A |
|---|---|
| 商品货号： | TS259274 |
| <strong>Cell line </strong>： | EFM-192A |
| DSMZ no. ： | TS259274 |
| Species ： | originally confirmed as human with IEF of AST, NP, MDH, later by STR typing |
| Cell type ： | breast carcinoma |
| Origin ： | established from the pleural effusion of the left side from a 46-year-old Caucasian woman with metastasizing breast adenocarcinoma in 1985; sister cell lines EFM-192B and C show differences in molecular aspects; erbB-2 gene is amplified and overexpressed |
| Reference(s) ： | 15156 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid, adherent growing as monolayer; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:2 to 1:4 once a week using trypsin/EDTA; seed out at ca. 2 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 5-8 days |
| Harvest ： | cell harvest of about 8-12 x 106 cells/ 80cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as EFM-192B/EFM-192C using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human flat-moded near-triploid karyotype with 20% polyploidy - 68(60-70)<3n>XXXX, +X, -1, -4, +9, -12, -17, -17, -18, -22, +6mar, add(1)(p22), add(1)(p?32), add(2)(q35/37), add(3)(q27/29), i(15q), add(17)(p12), dminx1 present at 38% |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
