RGE
RGE
规格:
货期:
编号:TS259283
品牌:Testobio
| 产品名称： | RGE |
|---|---|
| 商品货号： | TS259283 |
| <strong>Cell line </strong>： | RGE |
| DSMZ no. ： | TS259283 |
| Species ： | originally confirmed as rat with IEF and species PCR, and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | glomerular endothelial cells |
| Origin ： | established from freshly isolated glomeruli from kidneys of 6-week-old female Sprague-Dawley rats |
| Reference(s) ： | 14707 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing as monolayer (after confluency cells tend to grow in multilayers); image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:3 twice a week using trypsin/EDTA (cells are only lightly adherent and can be detached by tapping the flask; however, to prepare single cell suspension, trypsin/EDTA treatment is necessary); seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 17 hours |
| Harvest ： | cell harvest of about 40-50 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | rat hypertriploid karyotype with 6% polyploidy - 67(67-72)<3n> - corresponds to published karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | RGE |
|---|---|
| 商品货号： | TS259283 |
| <strong>Cell line </strong>： | RGE |
| DSMZ no. ： | TS259283 |
| Species ： | originally confirmed as rat with IEF and species PCR, and recently as Rattus norvegicus origin by COI DNA barcoding |
| Cell type ： | glomerular endothelial cells |
| Origin ： | established from freshly isolated glomeruli from kidneys of 6-week-old female Sprague-Dawley rats |
| Reference(s) ： | 14707 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing as monolayer (after confluency cells tend to grow in multilayers); image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:2 to 1:3 twice a week using trypsin/EDTA (cells are only lightly adherent and can be detached by tapping the flask; however, to prepare single cell suspension, trypsin/EDTA treatment is necessary); seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 17 hours |
| Harvest ： | cell harvest of about 40-50 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | rat hypertriploid karyotype with 6% polyploidy - 67(67-72)<3n> - corresponds to published karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
