PYS
PYS
规格:
货期:
编号:TS259322
品牌:Testobio
| 产品名称： | PYS |
|---|---|
| 商品货号： | TS259322 |
| <strong>Cell line </strong>： | PYS |
| DSMZ no. ： | TS259322 |
| Species ： | confirmed as murine with IEF of LDH, MDH, NP and by species PCR; carries a deletion in the DISC-1 gene, as reported for mice of the 129-inbred strain |
| Cell type ： | teratocarcinoma |
| Origin ： | the transplantable tumor OTT-6050 arose from a 6-day-old embryo grafted into the testis of a strain 129 mouse; this solid subcutaneous tumor was minced in PBS and mice were injected intraperitoneally; ascites containing embryoid bodies developed and cell cultures were initiated from the embryoid bodies |
| Reference(s) ： | 14711 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayers |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:5 to 1:10 every 2-3 days using trypsin/EDTA; seed out at ca. 2-3 x 10 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | murine hypotetraploid karyotype - 70(66-72)<4n> - 7-11 centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
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章丽敏
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朱静
18067106081 -
李晴
18067408036 -
李晶晶
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周雪
13372109301
| 产品名称： | PYS |
|---|---|
| 商品货号： | TS259322 |
| <strong>Cell line </strong>： | PYS |
| DSMZ no. ： | TS259322 |
| Species ： | confirmed as murine with IEF of LDH, MDH, NP and by species PCR; carries a deletion in the DISC-1 gene, as reported for mice of the 129-inbred strain |
| Cell type ： | teratocarcinoma |
| Origin ： | the transplantable tumor OTT-6050 arose from a 6-day-old embryo grafted into the testis of a strain 129 mouse; this solid subcutaneous tumor was minced in PBS and mice were injected intraperitoneally; ascites containing embryoid bodies developed and cell cultures were initiated from the embryoid bodies |
| Reference(s) ： | 14711 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayers |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:5 to 1:10 every 2-3 days using trypsin/EDTA; seed out at ca. 2-3 x 10 cells/80 cm2 |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | murine hypotetraploid karyotype - 70(66-72)<4n> - 7-11 centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |
