HPD-2NR
HPD-2NR
规格:
货期:
编号:TS259334
品牌:Testobio
| 产品名称： | HPD-2NR |
|---|---|
| 商品货号： | TS259334 |
| <strong>Cell line </strong>： | HPD-2NR |
| DSMZ no. ： | TS259334 |
| Species ： | originally confirmed as Syrian (Golden) Hamster by species PCR and recently as Mesocricetus auratus origin by COI DNA barcoding |
| Cell type ： | pancreas ductal adenocarcinoma |
| Origin ： | established from the pancreatic ductal adenocarcinoma of a female Syrian (Golden) hamster induced by treatment with BHP (chemical carcinogen); sister cell line of HPD-1NR (DSM ACC 314); described in the literature to be homotransplantable (i.e. in other syngeneic hamsters) and to carry a mutation in exon 1 of the c-Ki-ras oncogene |
| Reference(s) ： | 14784 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial cells growing as monolayers |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:8 every 2-6 days using trypsin/EDTA; seed out at about 0.4 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 1.5-2.0 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Fingerprint ： | unique and heterozygous DNA profile with pYNH24 single locus probe |
| Cytogenetics ： | near triploid highly rearranged karyotype with 29% polyploidy - 58-67<3n>XX, -X - hamster markers present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | HPD-2NR |
|---|---|
| 商品货号： | TS259334 |
| <strong>Cell line </strong>： | HPD-2NR |
| DSMZ no. ： | TS259334 |
| Species ： | originally confirmed as Syrian (Golden) Hamster by species PCR and recently as Mesocricetus auratus origin by COI DNA barcoding |
| Cell type ： | pancreas ductal adenocarcinoma |
| Origin ： | established from the pancreatic ductal adenocarcinoma of a female Syrian (Golden) hamster induced by treatment with BHP (chemical carcinogen); sister cell line of HPD-1NR (DSM ACC 314); described in the literature to be homotransplantable (i.e. in other syngeneic hamsters) and to carry a mutation in exon 1 of the c-Ki-ras oncogene |
| Reference(s) ： | 14784 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial cells growing as monolayers |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:8 every 2-6 days using trypsin/EDTA; seed out at about 0.4 x 105 cells/cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40 hours |
| Harvest ： | cell harvest of ca. 1.5-2.0 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Fingerprint ： | unique and heterozygous DNA profile with pYNH24 single locus probe |
| Cytogenetics ： | near triploid highly rearranged karyotype with 29% polyploidy - 58-67<3n>XX, -X - hamster markers present - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |
