MUTZ-3
MUTZ-3
规格:
货期:
编号:TS259344
品牌:Testobio
| 产品名称： | MUTZ-3 |
|---|---|
| 商品货号： | TS259344 |
| <strong>Cell line </strong>： | MUTZ-3 |
| DSMZ no. ： | TS259344 |
| Species ： | confirmed as human originally with IEF of AST, LDH, MDH, NP later by STR-typing |
| Cell type ： | acute myelomonocytic leukemia |
| Origin ： | established from the peripheral blood of a 29-year-old man with acute myelomonocytic leukemia (AML FAB M4) at diagnosis in 1993; this cell line is TSLP responsive and serves as dendritic cell model. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14482, 14919, 14918, 14618, 14722, 14739, 18187, 16556, 16670 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round blast-like cells growing as single cells and sometimes in small clumps in suspension; image |
| Medium ： | 60% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSM ACC 35) or purified cytokine (see references) |
| Subculture ： | cultivate in 24-well-plates (strongly recommended); seed out at ca. 1.0 x 106 cells/ml; viability may decrease within the first days and growth may start only 1-2 weeks after thawing; in growth phase split 1:2 every 4-6 days; maintain at 0.4-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 100-120 hours |
| Harvest ： | saturation density at 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD5 -, CD7 -, CD8 -, CD13 +, CD14 +, CD15 +, CD19 -, CD34 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 6% tetraploidy - 46(44-48)<2n>XY, t(1;3)(q43;q13)inv(3)(q21q26), t(2;7)(q36;q36)inv(7)(p15q36), t(12;22)(p13;q12) - carries t(12;22) recurrent in AML M4 - also carries masked inv(3) associated with abnormal megakaryocytopoiesis |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | MUTZ-3 |
|---|---|
| 商品货号： | TS259344 |
| <strong>Cell line </strong>： | MUTZ-3 |
| DSMZ no. ： | TS259344 |
| Species ： | confirmed as human originally with IEF of AST, LDH, MDH, NP later by STR-typing |
| Cell type ： | acute myelomonocytic leukemia |
| Origin ： | established from the peripheral blood of a 29-year-old man with acute myelomonocytic leukemia (AML FAB M4) at diagnosis in 1993; this cell line is TSLP responsive and serves as dendritic cell model. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14482, 14919, 14918, 14618, 14722, 14739, 18187, 16556, 16670 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round blast-like cells growing as single cells and sometimes in small clumps in suspension; image |
| Medium ： | 60% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSM ACC 35) or purified cytokine (see references) |
| Subculture ： | cultivate in 24-well-plates (strongly recommended); seed out at ca. 1.0 x 106 cells/ml; viability may decrease within the first days and growth may start only 1-2 weeks after thawing; in growth phase split 1:2 every 4-6 days; maintain at 0.4-1.0 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 100-120 hours |
| Harvest ： | saturation density at 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD5 -, CD7 -, CD8 -, CD13 +, CD14 +, CD15 +, CD19 -, CD34 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 6% tetraploidy - 46(44-48)<2n>XY, t(1;3)(q43;q13)inv(3)(q21q26), t(2;7)(q36;q36)inv(7)(p15q36), t(12;22)(p13;q12) - carries t(12;22) recurrent in AML M4 - also carries masked inv(3) associated with abnormal megakaryocytopoiesis |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
