CAL-51
CAL-51
规格:
货期:
编号:TS259350
品牌:Testobio
| 产品名称： | CAL-51 |
|---|---|
| 商品货号： | TS259350 |
| <strong>Cell line </strong>： | CAL-51 |
| DSMZ no. ： | TS259350 |
| Species ： | originally confirmed as human with IEF of AST, LDH, NP and recently by STR fingerprinting |
| Cell type ： | breast carcinoma |
| Origin ： | established from the pleural effusion metastasis of a 45-year-old woman with progressive breast adenocarcinoma (after radio-, chemotherapy and surgery) in 1985; rare example of tumor cell line with normal karyotype |
| Reference(s) ： | 14563 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing in monolayers; the culture contains usually a large amount of cell debris; image; image |
| Medium ： | 80-90% Dulbeccos MEM (4.5 g/L glucose) + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split culture 1:2 to 1:4 once or twice a week using trypsin/EDTA before cells reach complete confluency |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | 50-60 hours |
| Harvest ： | cell harvest of ca. 15-20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 28% tetraploidy - 46<2n>XX, no consistent abnormality detected - rare example of tumor cell line with normal karyotype - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
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李晶晶
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周雪
13372109301
| 产品名称： | CAL-51 |
|---|---|
| 商品货号： | TS259350 |
| <strong>Cell line </strong>： | CAL-51 |
| DSMZ no. ： | TS259350 |
| Species ： | originally confirmed as human with IEF of AST, LDH, NP and recently by STR fingerprinting |
| Cell type ： | breast carcinoma |
| Origin ： | established from the pleural effusion metastasis of a 45-year-old woman with progressive breast adenocarcinoma (after radio-, chemotherapy and surgery) in 1985; rare example of tumor cell line with normal karyotype |
| Reference(s) ： | 14563 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing in monolayers; the culture contains usually a large amount of cell debris; image; image |
| Medium ： | 80-90% Dulbeccos MEM (4.5 g/L glucose) + 10-20% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split culture 1:2 to 1:4 once or twice a week using trypsin/EDTA before cells reach complete confluency |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | 50-60 hours |
| Harvest ： | cell harvest of ca. 15-20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 28% tetraploidy - 46<2n>XX, no consistent abnormality detected - rare example of tumor cell line with normal karyotype - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
