HT-29
HT-29
规格:
货期:
编号:TS259354
品牌:Testobio
| 产品名称： | HT-29 |
|---|---|
| 商品货号： | TS259354 |
| <strong>Cell line </strong>： | HT-29 |
| DSMZ no. ： | TS259354 |
| Species ： | confirmed originally as human with IEF of AST, LDH |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described in the literature to be heterotransplantable forming well-differentiated grade I tumors |
| Reference(s) ： | 15135 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epitheloid cells growing as monolayers and in large colonies; image |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS |
| Subculture ： | seed out at about 2-3 x 106 cells into a 80 cm2 flask; split confluent culture 1:3 to 1:6 every 3-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40-60 hours |
| Harvest ： | cell harvest of about 25 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 17.5% polyploidy - 71(63-74)<3n>XX, -X, +1, +2, +7, -8, -9, +11, -13, -13, -14, +15, -18, +19, -21, -22, +4-6mar, del(3)(p24), del(4)(q22)x1-2, add(6)(q25), add(7)(p15), i(13q), add(19)(q13) - sideline with i(17q), t(17;19)(p10;q10) - resembles published karyotype and to that of CX-1 which is a subclone - carries very large isochromosomal ABR marker |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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周雪
13372109301
| 产品名称： | HT-29 |
|---|---|
| 商品货号： | TS259354 |
| <strong>Cell line </strong>： | HT-29 |
| DSMZ no. ： | TS259354 |
| Species ： | confirmed originally as human with IEF of AST, LDH |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described in the literature to be heterotransplantable forming well-differentiated grade I tumors |
| Reference(s) ： | 15135 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epitheloid cells growing as monolayers and in large colonies; image |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS |
| Subculture ： | seed out at about 2-3 x 106 cells into a 80 cm2 flask; split confluent culture 1:3 to 1:6 every 3-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40-60 hours |
| Harvest ： | cell harvest of about 25 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 17.5% polyploidy - 71(63-74)<3n>XX, -X, +1, +2, +7, -8, -9, +11, -13, -13, -14, +15, -18, +19, -21, -22, +4-6mar, del(3)(p24), del(4)(q22)x1-2, add(6)(q25), add(7)(p15), i(13q), add(19)(q13) - sideline with i(17q), t(17;19)(p10;q10) - resembles published karyotype and to that of CX-1 which is a subclone - carries very large isochromosomal ABR marker |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
