CPC-N
CPC-N
规格:
货期:
编号:TS259359
品牌:Testobio
| 产品名称： | CPC-N |
|---|---|
| 商品货号： | TS259359 |
| <strong>Cell line </strong>： | CPC-N |
| DSMZ no. ： | TS259359 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | small cell lung carcinoma |
| Origin ： | established from the pleural effusion metastasis of a patient with small cell lung carcinoma in 1987 (= CPN) |
| Reference(s) ： | 14838, 15133 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round cells growing in suspension in very large aggregates preventing exact estimation of cell numbers |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS |
| Subculture ： | split saturated culture 1:2 every 3-4 days; seed out 2 x 106 cells in 5 ml; viability remains at max. 60%, large amounts of cell debris may prevail in the flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 4 days |
| Harvest ： | maximal density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 16% polyploidy - 49(47-51)<2n>XY, +6, +15, +20, add(1)(p36), i(3q), del(6)(q1?3q2?5), der(8)t(8;17)(p23;q21), add(13)(p11)del(13)(q12q22), add(15)(p12), ins(16;8)(p13;HSR?q24) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | CPC-N |
|---|---|
| 商品货号： | TS259359 |
| <strong>Cell line </strong>： | CPC-N |
| DSMZ no. ： | TS259359 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | small cell lung carcinoma |
| Origin ： | established from the pleural effusion metastasis of a patient with small cell lung carcinoma in 1987 (= CPN) |
| Reference(s) ： | 14838, 15133 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly round cells growing in suspension in very large aggregates preventing exact estimation of cell numbers |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS |
| Subculture ： | split saturated culture 1:2 every 3-4 days; seed out 2 x 106 cells in 5 ml; viability remains at max. 60%, large amounts of cell debris may prevail in the flask |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 4 days |
| Harvest ： | maximal density at ca. 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 16% polyploidy - 49(47-51)<2n>XY, +6, +15, +20, add(1)(p36), i(3q), del(6)(q1?3q2?5), der(8)t(8;17)(p23;q21), add(13)(p11)del(13)(q12q22), add(15)(p12), ins(16;8)(p13;HSR?q24) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
