KPL-1
KPL-1
规格:
货期:
编号:TS259384
品牌:Testobio
| 产品名称： | KPL-1 |
|---|---|
| 商品货号： | TS259384 |
| <strong>Cell line </strong>： | KPL-1 |
| DSMZ no. ： | TS259384 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | breast carcinoma (derivative of MCF-7) |
| Origin ： | established from the pleural fluid of a 50-year-old Japanese woman with ductal carcinoma of the breast with a predominant intraductal component in 1992; cells were described in the literature to express estrogen, but not progesteron receptors and to secrete tissue polypeptide antigen; however, DNA fingerprinting analysis at the DSMZ showed cross-contamination with cell line MCF-7; MCF-7 was established from the pleural effusion of a 69-year-old Caucasian woman with metastatic mammary carcinoma |
| Reference(s) ： | 14698 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent growing in monolayers (very dense colonies) |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:4 to 1:8 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hypertriploid karyotype with 4% polyploidy - 77(70-82)<3n>XX, -X, -1, +2, -11, -13, +14, +15, -17, -18, -19, -20, -20, -21, -22, +15mar - add(X)(q28), add(2)(q35), add(3)(p25)x2, add(5)(p15), add(7)(p14), add(7)(p14), add(8)(p12), add(9)(p22), add(10)(p13)x2, add(19)(q13) - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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联系我们CONTACT US
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0574-87917803
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testobio@163.com
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浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
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联系方式
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | KPL-1 |
|---|---|
| 商品货号： | TS259384 |
| <strong>Cell line </strong>： | KPL-1 |
| DSMZ no. ： | TS259384 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | breast carcinoma (derivative of MCF-7) |
| Origin ： | established from the pleural fluid of a 50-year-old Japanese woman with ductal carcinoma of the breast with a predominant intraductal component in 1992; cells were described in the literature to express estrogen, but not progesteron receptors and to secrete tissue polypeptide antigen; however, DNA fingerprinting analysis at the DSMZ showed cross-contamination with cell line MCF-7; MCF-7 was established from the pleural effusion of a 69-year-old Caucasian woman with metastatic mammary carcinoma |
| Reference(s) ： | 14698 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent growing in monolayers (very dense colonies) |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:4 to 1:8 once or twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hypertriploid karyotype with 4% polyploidy - 77(70-82)<3n>XX, -X, -1, +2, -11, -13, +14, +15, -17, -18, -19, -20, -20, -21, -22, +15mar - add(X)(q28), add(2)(q35), add(3)(p25)x2, add(5)(p15), add(7)(p14), add(7)(p14), add(8)(p12), add(9)(p22), add(10)(p13)x2, add(19)(q13) - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
