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L-5178-Y
L-5178-Y
规格:
货期:
编号:TS259390
品牌:Testobio
产品名称: L-5178-Y
商品货号: TS259390
<strong>Cell line </strong>: L-5178-Y
DSMZ no. : TS259390
Species : originally confirmed as mouse with IEF of AST, LDH, MDH, NP and by species PCR and recently as mus musculus by COI DNA Barcoding
Cell type : T cell lymphoma
Origin : established from an 8-month-old female DBA/2 mouse with T cell lymphoma in 1985
Reference(s) : 14522, 14523
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: grows as single or aggregated round cells in suspension
Medium : 90% RPMI 1640 + 10% h.i. FBS
Subculture : seed out at 0.1-0.5 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 1-2 days
Incubation : at 37 °C with 5% CO2
Doubling time : about 20 hours
Harvest : max. density at about 2.0 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Cytogenetics : murine near diploid karyotype with 13% polyploidy - 39(37-42)<2n> - 1 centric fusion marker
Viruses : ELISA: reverse transcriptase positive; PCR: SMRV -
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L-5178-Y

  • 货号: TS259390
  • 好评
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  • 品牌 : TESTOBIO
产品名称: L-5178-Y
商品货号: TS259390
<strong>Cell line </strong>: L-5178-Y
DSMZ no. : TS259390
Species : originally confirmed as mouse with IEF of AST, LDH, MDH, NP and by species PCR and recently as mus musculus by COI DNA Barcoding
Cell type : T cell lymphoma
Origin : established from an 8-month-old female DBA/2 mouse with T cell lymphoma in 1985
Reference(s) : 14522, 14523
Biosafety level : 1
Permissions and restrictions : A
<strong>&nbsp;</strong>: DSMZ Scientific Data:
Morphology: grows as single or aggregated round cells in suspension
Medium : 90% RPMI 1640 + 10% h.i. FBS
Subculture : seed out at 0.1-0.5 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 1-2 days
Incubation : at 37 °C with 5% CO2
Doubling time : about 20 hours
Harvest : max. density at about 2.0 x 106 cells/ml
Storage : frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma : negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Cytogenetics : murine near diploid karyotype with 13% polyploidy - 39(37-42)<2n> - 1 centric fusion marker
Viruses : ELISA: reverse transcriptase positive; PCR: SMRV -
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