| 产品名称: | L-5178-Y |
|---|---|
| 商品货号: | TS259390 |
| <strong>Cell line </strong>: | L-5178-Y |
| DSMZ no. : | TS259390 |
| Species : | originally confirmed as mouse with IEF of AST, LDH, MDH, NP and by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type : | T cell lymphoma |
| Origin : | established from an 8-month-old female DBA/2 mouse with T cell lymphoma in 1985 |
| Reference(s) : | 14522, 14523 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | grows as single or aggregated round cells in suspension |
| Medium : | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture : | seed out at 0.1-0.5 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 1-2 days |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | about 20 hours |
| Harvest : | max. density at about 2.0 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics : | murine near diploid karyotype with 13% polyploidy - 39(37-42)<2n> - 1 centric fusion marker |
| Viruses : | ELISA: reverse transcriptase positive; PCR: SMRV - |
| 产品名称: | L-5178-Y |
|---|---|
| 商品货号: | TS259390 |
| <strong>Cell line </strong>: | L-5178-Y |
| DSMZ no. : | TS259390 |
| Species : | originally confirmed as mouse with IEF of AST, LDH, MDH, NP and by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type : | T cell lymphoma |
| Origin : | established from an 8-month-old female DBA/2 mouse with T cell lymphoma in 1985 |
| Reference(s) : | 14522, 14523 |
| Biosafety level : | 1 |
| Permissions and restrictions : | A |
| <strong> </strong>: | DSMZ Scientific Data: |
| Morphology: | grows as single or aggregated round cells in suspension |
| Medium : | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture : | seed out at 0.1-0.5 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 1-2 days |
| Incubation : | at 37 °C with 5% CO2 |
| Doubling time : | about 20 hours |
| Harvest : | max. density at about 2.0 x 106 cells/ml |
| Storage : | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma : | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics : | murine near diploid karyotype with 13% polyploidy - 39(37-42)<2n> - 1 centric fusion marker |
| Viruses : | ELISA: reverse transcriptase positive; PCR: SMRV - |