SK-MEL-3
SK-MEL-3
规格:
货期:
编号:TS259393
品牌:Testobio
| 产品名称： | SK-MEL-3 |
|---|---|
| 商品货号： | TS259393 |
| <strong>Cell line </strong>： | SK-MEL-3 |
| DSMZ no. ： | TS259393 |
| Species ： | confirmed as human with IEF of AST, LDH, MDH |
| Cell type ： | melanoma |
| Origin ： | established from the lymph node metastasis of a malignant melanoma from a 42-year-old Caucasian woman |
| Reference(s) ： | 15135 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial cells growing as monolayer |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS |
| Subculture ： | split confuent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2 in 8-10 ml medium |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-tetraploid karyotype with 3% polyploidy - 92(80-93)<4n>XX, -X, -X, -6, +7, +7, +7, -10, -10, -16, -16, +20, +20, +20, +20, +22, dup(7)(p15p22)x2, der(9)t(7;9)(p11;p21)dup(7)(p15p22)x2 - sideline with add(2)(p23) marker - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
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章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | SK-MEL-3 |
|---|---|
| 商品货号： | TS259393 |
| <strong>Cell line </strong>： | SK-MEL-3 |
| DSMZ no. ： | TS259393 |
| Species ： | confirmed as human with IEF of AST, LDH, MDH |
| Cell type ： | melanoma |
| Origin ： | established from the lymph node metastasis of a malignant melanoma from a 42-year-old Caucasian woman |
| Reference(s) ： | 15135 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial cells growing as monolayer |
| Medium ： | 90% McCoys 5A + 10% h.i. FBS |
| Subculture ： | split confuent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2 in 8-10 ml medium |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 40-50 hours |
| Harvest ： | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-tetraploid karyotype with 3% polyploidy - 92(80-93)<4n>XX, -X, -X, -6, +7, +7, +7, -10, -10, -16, -16, +20, +20, +20, +20, +22, dup(7)(p15p22)x2, der(9)t(7;9)(p11;p21)dup(7)(p15p22)x2 - sideline with add(2)(p23) marker - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |
