N3-36
N3-36
规格:
货期:
编号:TS259396
品牌:Testobio
| 产品名称： | N3-36 |
|---|---|
| 商品货号： | TS259396 |
| <strong>Cell line </strong>： | N3-36 |
| DSMZ no. ： | TS259396 |
| Species ： | originally confirmed as mouse with IEF of AST, LDH, MDH, NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | hybridoma (anti-fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgM) reacting with neonatal and adult fast fibers and myosins in rodent and human on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin |
| Reference(s) ： | 14444 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells, lightly adherent |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids |
| Subculture ： | split saturated culture 1:3 to 1:5 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 24 hours |
| Harvest ： | maximal density of ca. 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | secretion of a IgM monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid karyotype, 20% polyploidy - 78-90<4n> - carries large telocentric and two centric fusion markers |
| Viruses ： | ELISA: reverse transcriptase positive |
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| 产品名称： | N3-36 |
|---|---|
| 商品货号： | TS259396 |
| <strong>Cell line </strong>： | N3-36 |
| DSMZ no. ： | TS259396 |
| Species ： | originally confirmed as mouse with IEF of AST, LDH, MDH, NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | hybridoma (anti-fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgM) reacting with neonatal and adult fast fibers and myosins in rodent and human on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin |
| Reference(s) ： | 14444 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells, lightly adherent |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids |
| Subculture ： | split saturated culture 1:3 to 1:5 every 2-3 days; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 24 hours |
| Harvest ： | maximal density of ca. 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | secretion of a IgM monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid karyotype, 20% polyploidy - 78-90<4n> - carries large telocentric and two centric fusion markers |
| Viruses ： | ELISA: reverse transcriptase positive |
